ArticleThe Journal of cell biology2001
The nonhelical tail domain of keratin 14 promotes filament bundling and enhances the mechanical properties of keratin intermediate filaments in vitro.
Article in The Journal of cell biology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
What it found
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Who cites it
33 citing papers in PubMed, 70 citations in OpenAlex.
- Role of the nonhelical tailpiece of myosin-II in regulating filament architecture and function.The Journal of cell biology · 2026Article
- Structure and function of the keratin 17 tail domain associated with keratin intermediate filament organization.European journal of cell biology · 2025Article
- NAT10-mediated mRNA N4-acetylcytidine modification promotes bladder cancer progression.Clinical and translational medicine · 2022Article
- Witch Nails (Krt90whnl): A spontaneous mouse mutation affecting nail growth and development.PloS one · 2022Article
- Review
- Review
- High S100A2 expression in keratinocytes in patients with drug eruption.Scientific reports · 2021Article
- Modes of genetic adaptations underlying functional innovations in the rumen.Science China. Life sciences · 2021Article
- Mechanical loading of desmosomes depends on the magnitude and orientation of external stress.Nature communications · 2018Article
- Mechanical Properties of Intermediate Filament Proteins.Methods in enzymology · 2016Article
- The tail domain is essential but the head domain dispensable for C. elegans intermediate filament IFA-2 function.PloS one · 2015Article
- Binding Interactions of Keratin-Based Hair Fiber Extract to Gold, Keratin, and BMP-2.PloS one · 2015Article
- Complete Structure of an Epithelial Keratin Dimer: Implications for Intermediate Filament Assembly.PloS one · 2015Article
- Interaction of plectin with keratins 5 and 14: dependence on several plectin domains and keratin quaternary structure.The Journal of investigative dermatology · 2014Article
- Directed expression of a chimeric type II keratin partially rescues keratin 5-null mice.The Journal of biological chemistry · 2014Article
- Attractive interactions among intermediate filaments determine network mechanics in vitro.PloS one · 2014Article
- The multi-faceted role of the actin cap in cellular mechanosensation and mechanotransduction.Soft matter · 2013Article
- Mathematical modeling of the impact of actin and keratin filaments on keratinocyte cell spreading.Biophysical journal · 2012Article
- Evolution of the vertebrate beaded filament protein, Bfsp2; comparing the in vitro assembly properties of a "tailed" zebrafish Bfsp2 to its "tailless" human orthologue.Experimental eye research · 2012Article
- Multiple sites in αB-crystallin modulate its interactions with desmin filaments assembled in vitro.PloS one · 2011Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
Abstract
Keratin filaments arise from the copolymerization of type I and II sequences, and form a pancytoplasmic network that provides vital mechanical support to epithelial cells. Keratins 5 and 14 are expressed as a pair in basal cells of stratified epithelia, where they occur as bundled arrays of filaments. In vitro, bundles of K5-K14 filaments can be induced in the absence of cross-linkers, and exhibit enhanced resistance to mechanical strain. This property is not exhibited by copolymers of K5 and tailless K14, in which the nonhelical tail domain has been removed, or copolymers of K5 and K19, a type I keratin featuring a short tail domain. The purified K14 tail domain binds keratin filaments in vitro with specificity (kD approximately 2 microM). When transiently expressed in cultured cells, the K14 tail domain associates with endogenous keratin filaments. Utilization of the K14 tail domain as a bait in a yeast two-hybrid screen pulls out type I keratin sequences from a skin cDNA library. These data suggest that the tail domain of K14 contributes to the ability of K5-K14 filaments to self-organize into large bundles showing enhanced mechanical resilience in vitro.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.