ArticleThe Journal of general physiology2001
Gating and conductance properties of BK channels are modulated by the S9-S10 tail domain of the alpha subunit. A study of mSlo1 and mSlo3 wild-type and chimeric channels.
Article in The Journal of general physiology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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20 citing papers in PubMed.
- Markov models and long-term memory in ion channels: A contradiction in terms?Biophysical journal · 2025Article
- CryoEM structures of anion exchanger 1 capture multiple states of inward- and outward-facing conformations.Communications biology · 2022Article
- Article
- Cysteine residue 911 in C-terminal tail of human BK(Ca)α channel subunit is crucial for its activation by carbon monoxide.Pflugers Archiv : European journal of physiology · 2011Article
- Using a five-state model for fitting amplitude histograms from MaxiK channels: beta-distributions reveal more than expected.European biophysics journal : EBJ · 2009Article
- A structural motif in the C-terminal tail of slo1 confers carbon monoxide sensitivity to human BK Ca channels.Pflugers Archiv : European journal of physiology · 2008Article
- Modes of operation of the BKCa channel beta2 subunit.The Journal of general physiology · 2007Article
- Intra- and intersubunit cooperativity in activation of BK channels by Ca2+.The Journal of general physiology · 2006Article
- Slo3 K+ channels: voltage and pH dependence of macroscopic currents.The Journal of general physiology · 2006Article
- Ring of negative charge in BK channels facilitates block by intracellular Mg2+ and polyamines through electrostatics.The Journal of general physiology · 2006Article
- Opposite regulation of Slick and Slack K+ channels by neuromodulators.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2006Article
- Strengths and limits of Beta distributions as a means of reconstructing the true single-channel current in patch clamp time series with fast gating.The Journal of membrane biology · 2006Article
- Electrophysiological characterization of C-terminal Kv4 channel fusion proteins.Pflugers Archiv : European journal of physiology · 2005Article
- Ligand-dependent activation of Slo family channels is defined by interchangeable cytosolic domains.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2004Article
- Distributions-per-level: a means of testing level detectors and models of patch-clamp data.The Journal of membrane biology · 2004Article
- A ring of eight conserved negatively charged amino acids doubles the conductance of BK channels and prevents inward rectification.Proceedings of the National Academy of Sciences of the United States of America · 2003Article
- Gating mechanism of BK (Slo1) channels: so near, yet so far.The Journal of general physiology · 2003Review
- Slo1 tail domains, but not the Ca2+ bowl, are required for the beta 1 subunit to increase the apparent Ca2+ sensitivity of BK channels.The Journal of general physiology · 2002Article
- Stepwise contribution of each subunit to the cooperative activation of BK channels by Ca2+.Proceedings of the National Academy of Sciences of the United States of America · 2002Article
- Elimination of the BK(Ca) channel's high-affinity Ca(2+) sensitivity.The Journal of general physiology · 2002Article
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2 authors.
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Abstract
The COOH-terminal S9-S10 tail domain of large conductance Ca(2+)-activated K(+) (BK) channels is a major determinant of Ca(2+) sensitivity (Schreiber, M., A. Wei, A. Yuan, J. Gaut, M. Saito, and L. Salkoff. 1999. Nat. Neurosci. 2:416-421). To investigate whether the tail domain also modulates Ca(2+)-independent properties of BK channels, we explored the functional differences between the BK channel mSlo1 and another member of the Slo family, mSlo3 (Schreiber, M., A. Yuan, and L. Salkoff. 1998. J. Biol. Chem. 273:3509-3516). Compared with mSlo1 channels, mSlo3 channels showed little Ca(2+) sensitivity, and the mean open time, burst duration, gaps between bursts, and single-channel conductance of mSlo3 channels were only 32, 22, 41, and 37% of that for mSlo1 channels, respectively. To examine which channel properties arise from the tail domain, we coexpressed the core of mSlo1 with either the tail domain of mSlo1 or the tail domain of mSlo3 channels, and studied the single-channel currents. Replacing the mSlo1 tail with the mSlo3 tail resulted in the following: increased open probability in the absence of Ca(2+); reduced the Ca(2+) sensitivity greatly by allowing only partial activation by Ca(2+) and by reducing the Hill coefficient for Ca(2+) activation; decreased the voltage dependence approximately 28%; decreased the mean open time two- to threefold; decreased the mean burst duration three- to ninefold; decreased the single-channel conductance approximately 14%; decreased the K(d) for block by TEA(i) approximately 30%; did not change the minimal numbers of three to four open and five to seven closed states entered during gating; and did not change the major features of the dependency between adjacent interval durations. These observations support a modular construction of the BK channel in which the tail domain modulates the gating kinetics and conductance properties of the voltage-dependent core domain, in addition to determining most of the high affinity Ca(2+) sensitivity.
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