ArticleApplied and environmental microbiology2001
Efficient homolactic fermentation by Kluyveromyces lactis strains defective in pyruvate utilization and transformed with the heterologous LDH gene.
Article in Applied and environmental microbiology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 91 citations in OpenAlex.
- Engineering nonconventional yeasts to produce carboxylic acids: Advances and Prospects.Synthetic and systems biotechnology · 2027Review
- Native and Recombinant Yeast Producers of Lactic Acid: Characteristics and Perspectives.International journal of molecular sciences · 2025Review
- What are the 100 most cited fungal genera?Studies in mycology · 2024Article
- Just around the Corner: Advances in the Optimization of Yeasts and Filamentous Fungi for Lactic Acid Production.Journal of fungi (Basel, Switzerland) · 2024Review
- Metabolic Engineering and Adaptive Evolution for Efficient Production of l-Lactic Acid in Saccharomyces cerevisiae.Microbiology spectrum · 2022Article
- The revenge of Zygosaccharomyces yeasts in food biotechnology and applied microbiology.World journal of microbiology & biotechnology · 2021Review
- Mechanisms underlying lactic acid tolerance and its influence on lactic acid production inMicrobial cell (Graz, Austria) · 2021Review
- Statins interfere with the attachment ofJournal of enzyme inhibition and medicinal chemistry · 2020Article
- Lactic acid production - producing microorganisms and substrates sources-state of art.Heliyon · 2020Review
- Effect of Pyruvate Decarboxylase Knockout on Product Distribution Using Pichia pastoris (Komagataella phaffii) Engineered for Lactic Acid Production.Bioengineering (Basel, Switzerland) · 2018Article
- Model-based biotechnological potential analysis of Kluyveromyces marxianus central metabolism.Journal of industrial microbiology & biotechnology · 2017Article
- Engineered biosynthesis of biodegradable polymers.Journal of industrial microbiology & biotechnology · 2016Review
- L-lactic acid production by Aspergillus brasiliensis overexpressing the heterologous ldha gene from Rhizopus oryzae.Microbial cell factories · 2015Article
- Expression of Lactate Dehydrogenase in Aspergillus niger for L-Lactic Acid Production.PloS one · 2015Article
- Improving industrial yeast strains: exploiting natural and artificial diversity.FEMS microbiology reviews · 2014Review
- Effect of oxygenation and temperature on glucose-xylose fermentation in Kluyveromyces marxianus CBS712 strain.Microbial cell factories · 2014Article
- Production of L-lactic acid by the yeast Candida sonorensis expressing heterologous bacterial and fungal lactate dehydrogenases.Microbial cell factories · 2013Article
- Efficient production of L-lactic acid from xylose by Pichia stipitis.Applied and environmental microbiology · 2007Article
- Improvement of lactic acid production in Saccharomyces cerevisiae by cell sorting for high intracellular pH.Applied and environmental microbiology · 2006Article
- Lactate production yield from engineered yeasts is dependent from the host background, the lactate dehydrogenase source and the lactate export.Microbial cell factories · 2006Article
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A high yield of lactic acid per gram of glucose consumed and the absence of additional metabolites in the fermentation broth are two important goals of lactic acid production by microrganisms. Both purposes have been previously approached by using a Kluyveromyces lactis yeast strain lacking the single pyruvate decarboxylase gene (KlPDC1) and transformed with the heterologous lactate dehydrogenase gene (LDH). The LDH gene was placed under the control the KlPDC1 promoter, which has allowed very high levels of lactate dehydrogenase (LDH) activity, due to the absence of autoregulation by KlPdc1p. The maximal yield obtained was 0.58 g g(-1), suggesting that a large fraction of the glucose consumed was not converted into pyruvate. In a different attempt to redirect pyruvate flux toward homolactic fermentation, we used K. lactis LDH transformant strains deleted of the pyruvate dehydrogenase (PDH) E1alpha subunit gene. A great process improvement was obtained by the use of producing strains lacking both PDH and pyruvate decarboxylase activities, which showed yield levels of as high as 0.85 g g(-1) (maximum theoretical yield, 1 g g(-1)), and with high LDH activity.
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