ArticleApplied and environmental microbiology2001
Detection of hepatitis A virus by the nucleic acid sequence-based amplification technique and comparison with reverse transcription-PCR.
Article in Applied and environmental microbiology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
16 citing papers in PubMed, 91 citations in OpenAlex.
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- Viral outbreaks detection and surveillance using wastewater-based epidemiology, viral air sampling, and machine learning techniques: A comprehensive review and outlook.The Science of the total environment · 2022Review
- Recent Developments in Isothermal Amplification Methods for the Detection of Foodborne Viruses.Frontiers in microbiology · 2022Review
- Review on the contamination of wastewater by COVID-19 virus: Impact and treatment.The Science of the total environment · 2021Review
- Cascade of deoxyribozymes for the colorimetric analysis of drug resistance in Mycobacterium tuberculosis.Biosensors & bioelectronics · 2020Article
- Current and Future Perspectives on Isothermal Nucleic Acid Amplification Technologies for Diagnosing Infections.Infection and drug resistance · 2020Review
- Label-Free Pathogen Detection by a Deoxyribozyme Cascade with Visual Signal Readout.Sensors and actuators. B, Chemical · 2019Article
- Nucleic acid-based biotechnologies for food-borne pathogen detection using routine time-intensive culture-based methods and fast molecular diagnostics.Food science and biotechnology · 2016Review
- HAV in fresh vegetables: a hidden health risk in district Mardan, Pakistan.SpringerPlus · 2014Article
- Evaluation of the persistence of infectious human noroviruses on food surfaces by using real-time nucleic acid sequence-based amplification.Applied and environmental microbiology · 2008Article
- Diagnosis of hepatitis a virus infection: a molecular approach.Clinical microbiology reviews · 2006Review
- Real-time nucleic acid sequence-based amplification assay for detection of hepatitis A virus.Applied and environmental microbiology · 2005Article
- Isolation and detection of enterovirus RNA from large-volume water samples by using the NucliSens miniMAG system and real-time nucleic acid sequence-based amplification.Applied and environmental microbiology · 2005Article
- Multiplex nucleic acid sequence-based amplification for simultaneous detection of several enteric viruses in model ready-to-eat foods.Applied and environmental microbiology · 2004Article
- Combined immunomagnetic separation-molecular beacon-reverse transcription-PCR assay for detection of hepatitis A virus from environmental samples.Applied and environmental microbiology · 2004Article
- Development of Sensitive and Rapid RNA Transcription-based Isothermal Amplification Method for Detection ofAvicenna journal of medical biotechnologyArticle
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A nucleic acid sequence-based amplification (NASBA) technique for the detection of hepatitis A virus (HAV) in foods was developed and compared to the traditional reverse transcription (RT)-PCR technique. Oligonucleotide primers targeting the VP1 and VP2 genes encoding the major HAV capsid proteins were used for the amplification of viral RNA in an isothermal process resulting in the accumulation of RNA amplicons. Amplicons were detected by hybridization with a digoxigenin-labeled oligonucleotide probe in a dot blot assay format. Using the NASBA, as little as 0.4 ng of target RNA/ml was detected per comparison to 4 ng/ml for RT-PCR. When crude HAV viral lysate was used, a detection limit of 2 PFU (4 x 10(2) PFU/ml) was obtained with NASBA, compared to 50 PFU (1 x 10(4) PFU/ml) obtained with RT-PCR. No interference was encountered in the amplification of HAV RNA in the presence of excess nontarget RNA or DNA. The NASBA system successfully detected HAV recovered from experimentally inoculated samples of waste water, lettuce, and blueberries. Compared to RT-PCR and other amplification techniques, the NASBA system offers several advantages in terms of sensitivity, rapidity, and simplicity. This technique should be readily adaptable for detection of other RNA viruses in both foods and clinical samples.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.