ArticleApplied and environmental microbiology2001
In planta regulation of extension of an endophytic fungus and maintenance of high metabolic rates in its mycelium in the absence of apical extension.
Article in Applied and environmental microbiology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
19 citing papers in PubMed, 94 citations in OpenAlex.
- The Compatibility of theJournal of fungi (Basel, Switzerland) · 2026Article
- Article
- Elucidating the Molecular Mechanisms by which Seed-Borne Endophytic Fungi,International journal of molecular sciences · 2021Article
- Fungal Endophytic Community and Diversity Associated with Desert Shrubs Driven by Plant Identity and Organ Differentiation in Extremely Arid Desert Ecosystem.Journal of fungi (Basel, Switzerland) · 2021Article
- Global Changes in AsexualMicroorganisms · 2021Article
- Lolium perenne apoplast metabolomics for identification of novel metabolites produced by the symbiotic fungus Epichloë festucae.The New phytologist · 2020Article
- Fungal Alkaloid Occurrence in Endophyte-Infected Perennial Ryegrass during Seedling Establishment.Journal of chemical ecology · 2020Article
- Fungal Endophyte Colonization Patterns Alter Over Time in the Novel Association BetweenFrontiers in plant science · 2020Article
- VelA and LaeA are Key Regulators ofMicroorganisms · 2019Article
- A homologue of the fungal tetraspanin Pls1 is required for Epichloë festucae expressorium formation and establishment of a mutualistic interaction with Lolium perenne.Molecular plant pathology · 2019Article
- The endophytic symbiont Epichloë festucae establishes an epiphyllous net on the surface of Lolium perenne leaves by development of an expressorium, an appressorium-like leaf exit structure.The New phytologist · 2016Article
- cAMP Signaling Regulates Synchronised Growth of SymbioticFrontiers in plant science · 2016Article
- An endophyte of Picrorhiza kurroa Royle ex. Benth, producing menthol, phenylethyl alcohol and 3-hydroxypropionic acid, and other volatile organic compounds.World journal of microbiology & biotechnology · 2015Article
- Vegetative hyphal fusion and subsequent nuclear behavior in Epichloë grass endophytes.PloS one · 2015Article
- An extracellular siderophore is required to maintain the mutualistic interaction of Epichloë festucae with Lolium perenne.PLoS pathogens · 2013Article
- Fungal Endophyte Production of Reactive Oxygen Species is Critical for Maintaining the Mutualistic Symbiotic Interaction Between Epichloë festucae and Perennial Ryegrass.Plant signaling & behavior · 2007Article
- A p67Phox-like regulator is recruited to control hyphal branching in a fungal-grass mutualistic symbiosis.The Plant cell · 2006Article
- Effects of the fungal endophyte, Neotyphodium lolii, on net photosynthesis and growth rates of perennial ryegrass (Lolium perenne) are independent of In Planta endophyte concentration.Annals of botany · 2006Article
- Reactive oxygen species play a role in regulating a fungus-perennial ryegrass mutualistic interaction.The Plant cell · 2006Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The fungus Neotyphodium lolii is an endophytic symbiont. It grows in the intercellular spaces of the perennial ryegrass Lolium perenne, producing secondary metabolites which enhance the fitness of the association over that of uninfected L. perenne. We report that the average number of hyphal strands in a given section of a leaf remains constant during the life of a leaf, indicating synchrony of leaf and hyphal extension, including cessation of hyphal extension when leaf extension ceases. We used a constitutively expressed reporter gene as an indicator of the mycelium's metabolic activity during and after hyphal extension. Reporter gene activity decreased when the mycelium stopped extending in liquid culture but not in planta. This indicates that in planta endophyte hyphae remain metabolically highly active when extension has ceased and throughout the life of the leaf they are colonizing. The behavior of the fungus in planta indicates the existence of signaling pathways which (i) synchronize the extension of leaf and hypha by regulating hyphal extension, (ii) suppress hyphal branching, and (iii) stop apical extension of fungal hyphae, without reducing the mycelium's metabolic activity. These signals may be crucial for the symbiosis, by allowing the endophyte to switch the focus of its metabolic activity from extension to the production of secondary metabolites.
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