ReviewMolecular biotechnology2001
Understanding the art of producing protein and nonprotein molecules in Escherichia coli.
Review in Molecular biotechnology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
27 citing papers in PubMed.
- Synthetic Genetic Elements Enable Rapid Characterization of Inorganic Carbon Uptake Systems inACS synthetic biology · 2025Article
- The United States Food and Drug Administration's Platform Technology Designation to Expedite the Development of Drugs.Pharmaceutics · 2024Review
- Identification, purification, and pharmacological activity analysis of Desmodus rotundus salivary plasminogen activator alpha1 (DSPAα1) expressed in transgenic rabbit mammary glands.Transgenic research · 2022Article
- Identification of Optimal Expression Parameters and Purification of a Codon-Optimized Human GLIS1 Transcription Factor from Escherichia coli.Molecular biotechnology · 2022Article
- A genetic toolkit for co-expression of multiple proteins of diverse physiological implication.Biotechnology reports (Amsterdam, Netherlands) · 2021Article
- Synthetic biology toolkit for engineering Cupriviadus necator H16 as a platform for COBiotechnology for biofuels · 2021Review
- Computational design of highly stable and soluble alcohol dehydrogenase for NADPH regeneration.Bioresources and bioprocessing · 2021Article
- Stepwise optimization of recombinant protein production in Escherichia coli utilizing computational and experimental approaches.Applied microbiology and biotechnology · 2020Review
- Production of functional human CuZn-SOD and EC-SOD in bitransgenic cloned goat milk.Transgenic research · 2018Article
- Recombinant biosynthesis of bacterial cellulose in genetically modified Escherichia coli.Bioprocess and biosystems engineering · 2018Article
- Optimizing the expression of a Heterologous chitinase: A study of different promoters.Bioengineered · 2017Article
- Long-Term Stable and Tightly Controlled Expression of Recombinant Proteins in Antibiotics-Free Conditions.PloS one · 2016Article
- Magnetic nanoparticle hyperthermia induced cytosine deaminase expression in microencapsulated E. coli for enzyme-prodrug therapy.Journal of biotechnology · 2015Article
- mRNA secondary structure engineering of Thermobifida fusca endoglucanase (Cel6A) for enhanced expression in Escherichia coli.World journal of microbiology & biotechnology · 2015Article
- Codon optimization and factorial screening for enhanced soluble expression of human ciliary neurotrophic factor in Escherichia coli.BMC biotechnology · 2014Article
- Expression systems and species used for transgenic animal bioreactors.BioMed research international · 2013Article
- Industrial production of recombinant therapeutics in Escherichia coli and its recent advancements.Journal of industrial microbiology & biotechnology · 2012Review
- A strategy of gene overexpression based on tandem repetitive promoters in Escherichia coli.Microbial cell factories · 2012Article
- Secretion and proteolysis of heterologous proteins fused to the Escherichia coli maltose binding protein in Pichia pastoris.Protein expression and purification · 2010Article
- Novel antibiotic-free plasmid selection system based on complementation of host auxotrophy in the NAD de novo synthesis pathway.Applied and environmental microbiology · 2010Article
Corrections and comments
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Authors and funding
1 author.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The high-level production of functional proteins in E. coli is a very extense field of research in biotechnology. A number of important aspects to be considered in the initial design of an expression system and their interplay, were clear years ago. However, in recent times, strategies that go beyond transcription, translation, stability, vector, and strain choice, have been developed; so now expression of active peptides can be viewed as a more integrated process. Coexpression of protein subunits, foldases and chaperones, protein folding, location and purification schemes, metabolic engineering of the cell's central metabolism, and in vitro refolding strategies, are some of the novelties that are now available to aid in the success of an efficient expression system for active heterologous proteins. This review presents a compilation of the basic issues that influence the success in the production of protein and nonprotein products in Escherichia coli, as well as some general strategies designed to facilitate downstream process operations and improve biosynthesis yields.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.