ArticleBiophysical journal2001
Polylysine-induced 2H NMR-observable domains in phosphatidylserine/phosphatidylcholine lipid bilayers.
Article in Biophysical journal, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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17 citing papers in PubMed, 53 citations in OpenAlex.
- A simple method for poly-D-lysine coating to enhance adhesion and maturation of primary cortical neuron culturesFrontiers in cellular neuroscience · 2023Article
- Polycation-Anionic Lipid Membrane Interactions.Langmuir : the ACS journal of surfaces and colloids · 2020Article
- β2-Microglobulin amyloid fibril-induced membrane disruption is enhanced by endosomal lipids and acidic pH.PloS one · 2014Article
- Phosphatidylinositol-4,5-bisphosphate enhances anionic lipid demixing by the C2 domain of PKCα.PloS one · 2014Article
- How lipid headgroups sense the membrane environment: an application of ¹⁴N NMR.Biophysical journal · 2012Article
- Electrostatic field effects on membrane domain segregation and on lateral diffusion.Biophysical reviews · 2011Review
- Morphological changes of supported lipid bilayers induced by lysozyme: planar domain formation vs. multilayer stacking.Colloids and surfaces. B, Biointerfaces · 2010Article
- Structure and dynamics of the myristoyl lipid modification of SRC peptides determined by 2H solid-state NMR spectroscopy.Biophysical journal · 2009Article
- Biomembrane sensitivity to structural changes in bound polymers.Journal of the American Chemical Society · 2009Article
- Vesicle diffusion close to a membrane: intermembrane interactions measured with fluorescence correlation spectroscopy.Biophysical journal · 2008Article
- Lipid lateral segregation driven by diacyl cyclodextrin interactions at the membrane surface.Biophysical journal · 2007Article
- Interaction of poly(L-lysines) with negatively charged membranes: an FT-IR and DSC study.European biophysics journal : EBJ · 2007Article
- Poly-L-lysine-induced morphology changes in mixed anionic/zwitterionic and neat zwitterionic-supported phospholipid bilayers.Biophysical journal · 2006Article
- Probing perturbation of bovine lung surfactant extracts by albumin using DSC and 2H-NMR.Biophysical journal · 2006Article
- Domain formation induced by the adsorption of charged proteins on mixed lipid membranes.Biophysical journal · 2005Article
- Topographical pattern dynamics in passive adhesion of cell membranes.Biophysical journal · 2004Article
- Interaction of poly(L-lysine)-g-poly(ethylene glycol) with supported phospholipid bilayers.Biophysical journal · 2004Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The interaction of three polylysines, Lys(5) (N = 5), Lys(30) (N = 30), and Lys(100) (N = 100), where N is the number of lysine residues per chain, with phosphatidylserine-containing lipid bilayer membranes was investigated using 2H NMR spectroscopy. Lys(30) and Lys(100) added to multilamellar vesicles composed of (70:30) (mol:mol) mixtures of choline-deuterated 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) + 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoserine (POPS) produced two resolvable 2H NMR spectral components under conditions of low ionic strength and for cases where the global anionic lipid charge was in excess over the global cationic polypeptide charge. The intensities and quadrupolar splittings of the two spectral components were consistent with the existence of polylysine-bound domains enriched in POPS, in coexistence with polylysine-free domains depleted in POPS. Lys(5), however, yielded no 2H NMR resolvable domains. Increasing ionic strength caused domains to become diffuse and eventually dissipate entirely. At physiological salt concentrations, only Lys(100) yielded 2H NMR-resolvable domains. Therefore, under physiological conditions of ionic strength, pH, and anionic lipid bilayer content, and in the absence of other, e.g., hydrophobic, contributions to the binding free energy, the minimum number of lysine residues sufficient to produce spectroscopically resolvable POPS-enriched domains on the 2H NMR millisecond timescale may be fewer than 100, but is certainly greater than 30.
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