ArticleBiophysical journal2001
Discrimination of cardiac subcellular creatine kinase fluxes by NMR spectroscopy: a new method of analysis.
Article in Biophysical journal, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed.
- Mitochondrial dynamics in the adult cardiomyocytes: which roles for a highly specialized cell?Frontiers in physiology · 2013Article
- Sensitivity analysis of flux determination in heart by H₂ ¹⁸O -provided labeling using a dynamic Isotopologue model of energy transfer pathways.PLoS computational biology · 2012Article
- Modulation of energy transfer pathways between mitochondria and myofibrils by changes in performance of perfused heart.The Journal of biological chemistry · 2010Article
- Bidirectionality and compartmentation of metabolic fluxes are revealed in the dynamics of isotopomer networks.International journal of molecular sciences · 2009Review
- Metabolic compartmentation - a system level property of muscle cells: real problems of diffusion in living cells.International journal of molecular sciences · 2008Article
- Cardiac system bioenergetics: metabolic basis of the Frank-Starling law.The Journal of physiology · 2006Review
- Heterogeneity of ADP diffusion and regulation of respiration in cardiac cells.Biophysical journal · 2003Article
- Identification of subcellular energy fluxes by P NMR spectroscopy in the perfused heart: contractility induced modifications of energy transfer pathways.Molecular biology reports · 2002Article
- Modeling the energy transfer pathways. creatine kinase activities and heterogeneous distribution of ADP in the perfused heart.Molecular biology reports · 2002Article
- On the origin of intracellular compartmentation and organized metabolic systems.Molecular and cellular biochemistryReview
- Structural and functional adaptations of striated muscles to CK deficiency.Molecular and cellular biochemistryReview
- CK flux or direct ATP transfer: versatility of energy transfer pathways evidenced by NMR in the perfused heart.Molecular and cellular biochemistryReview
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3 authors.
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Abstract
A challenge in the understanding of creatine kinase (CK) fluxes reflected by NMR magnetization transfer in the perfused rat heart is the choice of a kinetic model of analysis. The complexity of the energetic pathways, due to the presence of adenosine triphosphate (ATP)-inorganic phosphate (Pi) exchange, of metabolite compartmentation and of subcellular localization of CK isozymes cannot be resolve from the sole information obtained from a single NMR protocol. To analyze multicompartment exchanges, we propose a new strategy based on the simultaneous analysis of four inversion transfer protocols. The time course of ATP and Phosphocreatine (PCr) magnetizations computed from the McConnell equations were adjusted to their experimental value for exchange networks of increasing complexity (up to six metabolite pools). Exchange schemes were selected by the quality of their fit and their consistency with data from other sources: the size of mitochondrial pools and the ATP synthesis flux. The consideration of ATP-Pi exchange and of ATP compartmentation were insufficient to describe the data. The most appropriate exchange scheme in our normoxic heart involved the discrimination of three specific CK activities (cytosolic, mitochondrial, and close to ATPases). At the present level of heart contractility, the energy is transferred from mitochondria to myofibrils mainly by PCr.
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