ArticleJournal of the American Society for Mass Spectrometry2001
Automatic analysis of hydrogen/deuterium exchange mass spectra of peptides and proteins using calculations of isotopic distributions.
Article in Journal of the American Society for Mass Spectrometry, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
What it found
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Who cites it
29 citing papers in PubMed.
- Probing protein-lipid interactions by hydrogen/deuterium exchange-mass spectrometry: advances, challenges and future directions.Chemical science · 2026Review
- The Deuterium Calculator: An Open-Source Tool for Hydrogen-Deuterium Exchange Mass Spectrometry Analysis.Journal of proteome research · 2023Article
- Advances in Hydrogen/Deuterium Exchange Mass Spectrometry and the Pursuit of Challenging Biological Systems.Chemical reviews · 2022Review
- Mass Spectrometry-Based Protein Footprinting for Higher-Order Structure Analysis: Fundamentals and Applications.Chemical reviews · 2020Review
- Computational methods in mass spectrometry-based structural proteomics for studying protein structure, dynamics, and interactions.Computational and structural biotechnology journal · 2020Review
- deMix: Decoding Deuterated Distributions from Heterogeneous Protein States via HDX-MS.Scientific reports · 2019Article
- Protein Footprinting Comes of Age: Mass Spectrometry for Biophysical Structure Assessment.Molecular & cellular proteomics : MCP · 2017Review
- Statistical Examination of the a and a + 1 Fragment Ions from 193 nm Ultraviolet Photodissociation Reveals Local Hydrogen Bonding Interactions.Journal of the American Society for Mass Spectrometry · 2016Article
- Analytical Aspects of Hydrogen Exchange Mass Spectrometry.Annual review of analytical chemistry (Palo Alto, Calif.) · 2015Review
- Hexicon 2: automated processing of hydrogen-deuterium exchange mass spectrometry data with improved deuteration distribution estimation.Journal of the American Society for Mass Spectrometry · 2014Article
- Comparison of the Mahalanobis distance and Pearson's χ² statistic as measures of similarity of isotope patterns.Journal of the American Society for Mass Spectrometry · 2014Article
- Analysis of overlapped and noisy hydrogen/deuterium exchange mass spectra.Journal of the American Society for Mass Spectrometry · 2013Article
- Considerations in the analysis of hydrogen exchange mass spectrometry data.Methods in molecular biology (Clifton, N.J.) · 2013Article
- HDX workbench: software for the analysis of H/D exchange MS data.Journal of the American Society for Mass Spectrometry · 2012Article
- Improved sequence resolution by global analysis of overlapped peptides in hydrogen/deuterium exchange mass spectrometry.Journal of the American Society for Mass Spectrometry · 2012Article
- ExMS: data analysis for HX-MS experiments.Journal of the American Society for Mass Spectrometry · 2011Article
- Methods for the Analysis of High Precision Differential Hydrogen Deuterium Exchange Data.International journal of mass spectrometry · 2011Article
- Differential hydrogen/deuterium exchange mass spectrometry analysis of protein-ligand interactions.Expert review of proteomics · 2011Review
- Bayesian Peptide Peak Detection for High Resolution TOF Mass Spectrometry.IEEE transactions on signal processing : a publication of the IEEE Signal Processing Society · 2010Article
- Quality control based on isotopic distributions for high-throughput MALDI-TOF and MALDI-FTICR serum peptide profiling.Journal of the American Society for Mass Spectrometry · 2010Article
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
High mass-resolving power has been shown to be useful for studying the conformational dynamics of proteins by hydrogen/deuterium (H/D) exchange. A computer algorithm was developed that automatically identifies peptides and their extent of deuterium incorporation from H/D exchange mass spectra of enzymatic digests or fragment ions produced by collisionally induced dissociation (CID) or electron capture dissociation (ECD). The computer algorithm compares measured and calculated isotopic distributions and uses a fast calculation of isotopic distributions using the fast Fourier transform (FFT). The algorithm facilitates rapid and automated analysis of H/D exchange mass spectra suitable for high-throughput approaches to the study of peptide and protein structures. The algorithm also makes the identification independent on comparisons with undeuterated control samples. The applicability of the algorithm was demonstrated on simulated isotopic distributions as well as on experimental data, such as Fourier transform ion cyclotron resonance (FTICR) mass spectra of myoglobin peptic digests, and CID and ECD spectra of substance P.
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Registered trials
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