ArticleThe Journal of biological chemistry2002
Inhibition of protein-tyrosine phosphatase stimulates the dynamin-dependent endocytosis of ROMK1.
Article in The Journal of biological chemistry, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed, 56 citations in OpenAlex.
- Mechanistic target of rapamycin complex-1 plays a role in inhibiting kidney KAmerican journal of physiology. Renal physiology · 2026Article
- The endocytic adaptor ARH facilitates potassium conservation by regulating ROMK and BK.American journal of physiology. Renal physiology · 2025Article
- ROMK channels are inhibited in the aldosterone-sensitive distal nephron of renal tubule Nedd4-2-deficient mice.American journal of physiology. Renal physiology · 2022Article
- Thick Ascending Limb Sodium Transport in the Pathogenesis of Hypertension.Physiological reviews · 2019Review
- Romk1 Knockout Mice Do Not Produce Bartter Phenotype but Exhibit Impaired K Excretion.The Journal of biological chemistry · 2016Article
- Src-family protein tyrosine kinase phosphorylates WNK4 and modulates its inhibitory effect on KCNJ1 (ROMK).Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- MicroRNA-194 (miR-194) regulates ROMK channel activity by targeting intersectin 1.American journal of physiology. Renal physiology · 2014Article
- Src family protein tyrosine kinase regulates the basolateral K channel in the distal convoluted tubule (DCT) by phosphorylation of KCNJ10 protein.The Journal of biological chemistry · 2013Article
- Regulation of potassium channel trafficking in the distal nephron.Current opinion in nephrology and hypertension · 2013Review
- Protein phosphatase 1 modulates the inhibitory effect of With-no-Lysine kinase 4 on ROMK channels.American journal of physiology. Renal physiology · 2012Article
- MicroRNA 802 stimulates ROMK channels by suppressing caveolin-1.Journal of the American Society of Nephrology : JASN · 2011Article
- Angiotensin II diminishes the effect of SGK1 on the WNK4-mediated inhibition of ROMK1 channels.Kidney international · 2011Article
- Decrease in dietary K intake stimulates the generation of superoxide anions in the kidney and inhibits K secretory channels in the CCD.American journal of physiology. Renal physiology · 2010Article
- POSH stimulates the ubiquitination and the clathrin-independent endocytosis of ROMK1 channels.The Journal of biological chemistry · 2009Article
- A comprehensive guide to the ROMK potassium channel: form and function in health and disease.American journal of physiology. Renal physiology · 2009Review
- Regulation of wild-type and mutant KCNQ1/KCNE1 channels by tyrosine kinase.Pflugers Archiv : European journal of physiology · 2009Article
- Regulation of potassium (K) handling in the renal collecting duct.Pflugers Archiv : European journal of physiology · 2009Review
- Protein kinase C dependent inhibition of the heteromeric Kir4.1-Kir5.1 channel.Biochimica et biophysica acta · 2007Article
- Role of gp91phox -containing NADPH oxidase in mediating the effect of K restriction on ROMK channels and renal K excretion.Journal of the American Society of Nephrology : JASN · 2007Article
- Effect of hydrogen peroxide on ROMK channels in the cortical collecting duct.American journal of physiology. Renal physiology · 2007Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
We have previously shown that inhibiting protein-tyrosine kinase increased whereas inhibiting protein-tyrosine phosphatase (PTP) decreased renal outer medullary potassium channel 1 (ROMK1) channel activity (1). We have now used confocal microscopy, the patch clamp technique, and biotin labeling to further examine the role of tyrosine phosphorylation in regulating ROMK1 trafficking. Human embryonic kidney 293 cells were cotransfected with c-Src and green fluorescent protein-ROMK1, which has the same biophysical properties as those of ROMK1. Patch clamp studies have shown that phenylarsine oxide (PAO), an inhibitor of PTP, decreased the activity of ROMK1. Moreover, addition of PAO reduced the cell surface localization of green fluorescent protein-ROMK1 detected by confocal microscopy and diminished the surface ROMK1 density by 65% measured by biotin labeling. Also, PAO treatment significantly increased the phosphorylation of ROMK1. The notion that the effect of PAO is mediated by stimulating tyrosine phosphorylation-induced endocytosis of ROMK1 has also been supported by findings that mutating the tyrosine residue 337 of ROMK1 to alanine abolished the effect of PAO. Finally, the inhibitory effect of PAO on ROMK1 was completely blocked in the cells co-transfected with dominant negative dynamin (dynaminK44A). This indicates that the tyrosine phosphorylation-induced endocytosis of ROMK1 is dynamin-dependent. We conclude that inhibiting PTP increases ROMK1 phosphorylation and results in a dynamin-dependent internalization of the channel.
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