ArticleProceedings of the National Academy of Sciences of the United States of America2001
Structural basis for autoantibody recognition of phosphatidylserine-beta 2 glycoprotein I and apoptotic cells.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
31 citing papers in PubMed, 103 citations in OpenAlex.
- Editorial: Structure, isotypes, targets, and post-translational modifications of immunoglobulins and their role in infection, inflammation and autoimmunity, Volume II.Frontiers in immunology · 2022Article
- Restriction of the Global IgM Repertoire in Antiphospholipid Syndrome.Frontiers in immunology · 2022Article
- Anti-βClinical and experimental immunology · 2019Article
- Cellular and Molecular Mechanisms of Anti-Phospholipid Syndrome.Frontiers in immunology · 2018Review
- B-CD8Journal of immunology research · 2017Article
- Self-Antigen-Driven Thymic B Cell Class Switching Promotes T Cell Central Tolerance.Cell reports · 2016Article
- The analysis of clonal expansions in normal and autoimmune B cell repertoires.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2015Review
- An entity evolving into a community: defining the common ancestor and evolutionary trajectory of chronic lymphocytic leukemia stereotyped subset #4.Molecular medicine (Cambridge, Mass.) · 2015Article
- Clearance of Apoptotic Bodies, NETs, and Biofilm DNA: Implications for Autoimmunity.Frontiers in immunology · 2014Review
- Molecular basis of 9G4 B cell autoreactivity in human systemic lupus erythematosus.Journal of immunology (Baltimore, Md. : 1950) · 2013Article
- Vitellogenin recognizes cell damage through membrane binding and shields living cells from reactive oxygen species.The Journal of biological chemistry · 2013Article
- Temporal dynamics of clonal evolution in chronic lymphocytic leukemia with stereotyped IGHV4-34/IGKV2-30 antigen receptors: longitudinal immunogenetic evidence.Molecular medicine (Cambridge, Mass.) · 2013Article
- Antibodies that bind complex glycosaminoglycans accumulate in the Golgi.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Natural IgG autoantibodies are abundant and ubiquitous in human sera, and their number is influenced by age, gender, and disease.PloS one · 2013Article
- Protective Roles of Natural IgM Antibodies.Frontiers in immunology · 2012Article
- Induction of Mitochondria-Mediated Apoptosis in Ca Ski Human Cervical Cancer Cells Triggered by Mollic Acid Arabinoside Isolated from Leea indica.Evidence-based complementary and alternative medicine : eCAM · 2012Article
- The consequences of apoptosis in autoimmunity.Journal of autoimmunity · 2008Review
- Autoreactive IgG memory antibodies in patients with systemic lupus erythematosus arise from nonreactive and polyreactive precursors.Proceedings of the National Academy of Sciences of the United States of America · 2008Article
- Editing and escape from editing in anti-DNA B cells.Proceedings of the National Academy of Sciences of the United States of America · 2008Article
- Lupus nephritis: the central role of nucleosomes revealed.The American journal of pathology · 2008Review
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Apoptotic cells contain nuclear autoantigens that may initiate a systemic autoimmune response. To explore the mechanism of antibody binding to apoptotic cells, 3H9, a murine autoantibody with dual specificity for phospholipids and DNA, was used. H chain mutants of 3H9 were constructed, expressed as single-chain Fv (scFv) in Escherichia coli, and assessed for binding to phosphatidylserine, an antigen expressed on apoptotic cells. Both 3H9 and its germline revertant bound to dioleoyl phosphatidylserine in ELISA, and binding was enhanced by beta 2 glycoprotein I (beta 2GPI), a plasma protein that selectively binds to apoptotic cells. Higher relative affinity for DOPS-beta 2GPI was achieved by the introduction of Arg residues into the 3H9 H chain variable region at positions previously shown to mediate DNA binding. Specificity of the two structurally most diverse scFv for apoptotic cells was shown by flow cytometry, and two populations of scFv-bound cells were identified by differences in propidium iodide staining. The results suggest that, in autoimmunity, B cells with Ig receptors for apoptotic cells and DNA are positively selected, and that the antibodies they produce have the potential to affect the clearance and processing of apoptotic cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.