ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience2001
A functional role for intra-axonal protein synthesis during axonal regeneration from adult sensory neurons.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 187 papers.
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Who cites it
187 citing papers in PubMed, 338 citations in OpenAlex.
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- RBP-Driven RNA sorting and local translation establish the molecular identities of individual sensory axons.Nature communications · 2026Article
- An axon-intrinsic loop restricts nerve regeneration through axonal protein synthesis.Science advances · 2026Article
- KHSRP-mediated decay of axonally localized prenyl-Cdc42 mRNA slows nerve regeneration.PLoS genetics · 2025Article
- Glycation in Alzheimer's Disease and Type 2 Diabetes: The Prospect of Dual Drug Approaches for Therapeutic Interventions.Molecular neurobiology · 2025Review
- Cell and molecular profiles in peripheral nerves shift toward inflammatory phenotypes in diabetic peripheral neuropathy.The Journal of clinical investigation · 2025Article
- Regulation of Subcellular Protein Synthesis for Restoring Neural Connectivity.International journal of molecular sciences · 2025Review
- Disruption of G3BP1 granules promotes mammalian CNS and PNS axon regeneration.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Deciphering the molecular landscape of human peripheral nerves: implications for diabetic peripheral neuropathy.bioRxiv : the preprint server for biology · 2024Article
- Correlation of TRPA1 RNAscope and Agonist Responses.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2024Article
- Control of Selective mRNA Translation in Neuronal Subcellular Compartments in Health and Disease.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2023Review
- Experimental upregulation of developmentally downregulated ribosomal protein large subunits 7 and 7A promotes axon regeneration after injury in vivo.Experimental neurology · 2023Article
- The brain's dark transcriptome: Sequencing RNA in distal compartments of neurons and glia.Current opinion in neurobiology · 2023Review
- Cytosolic Ptbp2 modulates axon growth in motoneurons through axonal localization and translation of Hnrnpr.Nature communications · 2023Article
- Profiling Locally Translated mRNAs in Regenerating Axons.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Overexpressing eukaryotic elongation factor 1 alpha (eEF1A) proteins to promote corticospinal axon repair after injury.Cell death discovery · 2022Article
- Article
- Intra-axonal translation of Khsrp mRNA slows axon regeneration by destabilizing localized mRNAs.Nucleic acids research · 2022Article
- RSK1 promotes mammalian axon regeneration by inducing the synthesis of regeneration-related proteins.PLoS biology · 2022Article
- High-throughput translational profiling with riboPLATE-seq.Scientific reports · 2022Article
127 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Although intradendritic protein synthesis has been documented in adult neurons, the question of whether axons actively synthesize proteins remains controversial. Adult sensory neurons that are conditioned by axonal crush can rapidly extend processes in vitro by regulating the translation of existing mRNAs (Twiss et al., 2000). These regenerating processes contain axonal but not dendritic proteins. Here we show that these axonal processes of adult sensory neurons cultured after conditioning injury contain ribosomal proteins, translational initiation factors, and rRNA. Pure preparations of regenerating axons separated from the DRG cell bodies can actively synthesize proteins in vitro and contain ribosome-bound beta-actin and neurofilament mRNAs. Blocking protein synthesis in these regenerating sensory axons causes a rapid retraction of their growth cones when communication with the cell body is blocked by axotomy or colchicine treatment. These findings indicate that axons of adult mammalian neurons can synthesize proteins and suggest that, under some circumstances, intra-axonal translation contributes to structural integrity of the growth cone in regenerating axons. By immunofluorescence, translation factors, ribosomal proteins, and rRNA were also detected in motor axons of ventral spinal roots analyzed after 7 d in vivo after a peripheral axonal crush injury. Thus, adult motor neurons are also likely capable of intra-axonal protein synthesis in vivo after axonal injury.
Indexed as
Identifiers
11717363PMC6763927W2161365864What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.