ArticleJournal of bacteriology2001
Differential roles of the Pseudomonas aeruginosa PA14 rpoN gene in pathogenicity in plants, nematodes, insects, and mice.
Article in Journal of bacteriology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 51 papers.
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Who cites it
51 citing papers in PubMed.
- Retrospective analysis of secreted PrpL protease activity in clinical isolates ofFrontiers in microbiology · 2026Article
- Understanding the pathophysiology ofBurns & trauma · 2025Review
- The balance between antibiotic resistance and fitness/virulence inFrontiers in microbiology · 2023Review
- RpoN/Sfa2-dependent activation of the Pseudomonas aeruginosa H2-T6SS and its cognate arsenal of antibacterial toxins.Nucleic acids research · 2022Article
- Global Proteomic Profiling ofMicroorganisms · 2020Article
- Genome-Scale Mapping Reveals Complex Regulatory Activities of RpoN in Yersinia pseudotuberculosis.mSystems · 2020Article
- Animal infection models using non-mammals.Microbiology and immunology · 2020Review
- Potentiation of Aminoglycoside Lethality by CAntimicrobial agents and chemotherapy · 2019Article
- Defining the core essential genome ofProceedings of the National Academy of Sciences of the United States of America · 2019Article
- RpoN-Dependent Direct Regulation of Quorum Sensing and the Type VI Secretion System in Pseudomonas aeruginosa PAO1.Journal of bacteriology · 2018Article
- Cannibalism as a Possible Entry Route for Opportunistic Pathogenic Bacteria to Insect Hosts, Exemplified byInsects · 2018Article
- Targeting the alternative sigma factor RpoN to combat virulence in Pseudomonas aeruginosa.Scientific reports · 2017Article
- Functional modules of sigma factor regulons guarantee adaptability and evolvability.Scientific reports · 2016Article
- RpoN Regulates Virulence Factors of Pseudomonas aeruginosa via Modulating the PqsR Quorum Sensing Regulator.International journal of molecular sciences · 2015Article
- Mutations in RNA Polymerase Bridge Helix and Switch Regions Affect Active-Site Networks and Transcript-Assisted Hydrolysis.Journal of molecular biology · 2015Article
- Elucidation of sigma factor-associated networks in Pseudomonas aeruginosa reveals a modular architecture with limited and function-specific crosstalk.PLoS pathogens · 2015Article
- Role of Burkholderia pseudomallei Sigma N2 in Amino Acids Utilization and in Regulation of Catalase E Expression at the Transcriptional Level.International journal of bacteriology · 2015Article
- Comparative secretomics reveals novel virulence-associated factors of Vibrio parahaemolyticus.Frontiers in microbiology · 2015Article
- Lactonase-expressing Lactobacillus plantarum NC8 attenuates the virulence factors of multiple drug resistant Pseudomonas aeruginosa in co-culturing environment.World journal of microbiology & biotechnology · 2014Article
- Identification of the alternative sigma factor SigX regulon and its implications for Pseudomonas aeruginosa pathogenicity.Journal of bacteriology · 2014Article
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Authors and funding
5 authors.
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Abstract
We cloned the rpoN (ntrA, glnF) gene encoding the alternate sigma factor sigma(54) from the opportunistic multihost pathogen Pseudomonas aeruginosa strain PA14. A marker exchange protocol was used to construct the PA14 rpoN insertional mutation rpoN::Gen(r). PA14 rpoN::Gen(r) synthesized reduced levels of pyocyanin and displayed a variety of phenotypes typical of rpoN mutants, including a lack of motility and the failure to grow on nitrate, glutamate, or histidine as the sole nitrogen source. Compared to wild-type PA14, rpoN::Gen(r) was ca. 100-fold less virulent in a mouse thermal injury model and was significantly impaired in its ability to kill the nematode Caenorhabditis elegans. In an Arabidopsis thaliana leaf infectivity assay, although rpoN::Gen(r) exhibited significantly reduced attachment to trichomes, stomata, and the epidermal cell surface, did not attach perpendicularly to or perforate mesophyll cell walls, and proliferated less rapidly in Arabidopsis leaves, it nevertheless elicited similar disease symptoms to wild-type P. aeruginosa PA14 at later stages of infection. rpoN::Gen(r) was not impaired in virulence in a Galleria mellonella (greater wax moth) pathogenicity model. These data indicate that rpoN does not regulate the expression of any genes that encode virulence factors universally required for P. aeruginosa pathogenicity in diverse hosts.
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