ArticleThe Biochemical journal2001
Identification of a new polyphosphoinositide in plants, phosphatidylinositol 5-monophosphate (PtdIns5P), and its accumulation upon osmotic stress.
Article in The Biochemical journal, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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Who cites it
40 citing papers in PubMed, 108 citations in OpenAlex.
- Phosphoinositides in membrane remodeling during infections and cellular stresses.FEBS letters · 2026Review
- Plant PI-PLC signaling in stress and development.Plant physiology · 2025Review
- Phosphoinositides in plant-pathogen interaction: trends and perspectives.Stress biology · 2023Review
- Genomic identification of cottonFrontiers in plant science · 2023Article
- Chlamydomonas proteases: classification, phylogeny, and molecular mechanisms.Journal of experimental botany · 2021Article
- Link between Lipid Second Messengers and Osmotic Stress in Plants.International journal of molecular sciences · 2021Review
- Signalling Pinpointed to the Tip: The Complex Regulatory Network That Allows Pollen Tube Growth.Plants (Basel, Switzerland) · 2020Review
- PI5P and PI(3,5)PCell structure and function · 2017Review
- An Autophagy-Related Kinase Is Essential for the Symbiotic Relationship betweenThe Plant cell · 2016Article
- Role of Arabidopsis AtPI4Kγ3, a type II phosphoinositide 4-kinase, in abiotic stress responses and floral transition.Plant biotechnology journal · 2016Article
- The basal transcription complex component TAF3 transduces changes in nuclear phosphoinositides into transcriptional output.Molecular cell · 2015Article
- Involvement of Phosphatidylinositol 3-kinase in the regulation of proline catabolism in Arabidopsis thaliana.Frontiers in plant science · 2014Article
- PtdIns5P: news and views of its appearance, disappearance and deeds.Archives of biochemistry and biophysics · 2013Review
- Phosphatidylinositol 3-kinase plays a vital role in regulation of rice seed vigor via altering NADPH oxidase activity.PloS one · 2012Article
- The salt stress-induced LPA response in Chlamydomonas is produced via PLA₂ hydrolysis of DGK-generated phosphatidic acid.Journal of lipid research · 2011Article
- The nucleophosmin-anaplastic lymphoma kinase oncogene interacts, activates, and uses the kinase PIKfyve to increase invasiveness.The Journal of biological chemistry · 2011Article
- Phosphatidylinositol 5-phosphate links dehydration stress to the activity of ARABIDOPSIS TRITHORAX-LIKE factor ATX1.PloS one · 2010Article
- Identification and structural characterization of FYVE domain-containing proteins of Arabidopsis thaliana.BMC plant biology · 2010Article
- The Arabidopsis stem cell factor POLTERGEIST is membrane localized and phospholipid stimulated.The Plant cell · 2010Article
- Biochemical characterization of two wheat phosphoethanolamine N-methyltransferase isoforms with different sensitivities to inhibition by phosphatidic acid.The Journal of biological chemistry · 2009Article
Corrections and comments
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Authors and funding
6 authors at 4 institutions in 2 countries.
Funding
Abstract
Polyphosphoinositides play an important role in membrane trafficking and cell signalling. In plants, two PtdInsP isomers have been described, PtdIns3P and PtdIns4P. Here we report the identification of a third, PtdIns5P. Evidence is based on the conversion of the endogenous PtdInsP pool into PtdIns(4,5)P(2) by a specific PtdIns5P 4-OH kinase, and on in vivo (32)P-labelling studies coupled to HPLC head-group analysis. In Chlamydomonas, 3-8% of the PtdInsP pool was PtdIns5P, 10-15% was PtdIns3P and the rest was PtdIns4P. In seedlings of Vicia faba and suspension-cultured tomato cells, the level of PtdIns5P was about 18%, indicating that PtdIns5P is a general plant lipid that represents a significant proportion of the PtdInsP pool. Activating phospholipase C (PLC) signalling in Chlamydomonas cells with mastoparan increased the turnover of PtdIns(4,5)P(2) at the cost of PtdIns4P, but did not affect the level of PtdIns5P. This indicates that PtdIns(4,5)P(2) is synthesized from PtdIns4P rather than from PtdIns5P during PLC signalling. However, when cells were subjected to hyperosmotic stress, PtdIns5P levels rapidly increased, suggesting a role in osmotic-stress signalling. The potential pathways of PtdIns5P formation are discussed.
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