Evidence map›Paper›PMID 11716775›Full record

ArticleThe Biochemical journal2001

Regulation of type-II collagen gene expression during human chondrocyte de-differentiation and recovery of chondrocyte-specific phenotype in culture involves Sry-type high-mobility-group box (SOX) transcription factors.

D G Stokes, G Liu, R Dharmavaram, D Hawkins, S Piera-Velazquez, S A Jimenez

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The Biochemical journal, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 60 papers.

0numbers the graph read from it
0cells of the map it votes in
60citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

60 citing papers in PubMed.

  1. Article
  2. Review
  3. Article
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  5. Article
  6. Review
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  9. Review
  10. Article
  11. Anti-inflammatory capacity of Apremilast in human chondrocytes is dependent on SOX-9.Inflammation research : official journal of the European Histamine Research Society ... [et al.] · 2020
    Article
  12. Article
  13. Review
  14. Article
  15. Article
  16. Article
  17. Article
  18. Article
  19. [Andrographolide-releasing collagen scaffold enhance the ability of chondrocytes to maintain their specific phenotype under inflammatory environmentSheng wu yi xue gong cheng xue za zhi = Journal of biomedical engineering = Shengwu yixue gongchengxue zazhi · 2018
    Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

D G StokesDepartment of Medicine, Division of Rheumatology, 233 S. 10th Street, Thomas Jefferson University, Philadelphia, PA 19107, USA.
G Liu
R Dharmavaram
D Hawkins
S Piera-Velazquez
S A Jimenez

Funding

MOLECULAR CHARACTERIZATION OF FAMILIAL CHONDROCALCINOSISP01AR039740 · NIAMS · THOMAS JEFFERSON UNIVERSITY · PI JIMENEZ, SERGIO A · 1989 to 2006
$6.2M
NIAMS NIH HHS AR-39740
6 · The paper itself

Abstract

During ex vivo growth as monolayer cultures, chondrocytes proliferate and undergo a process of de-differentiation. This process involves a change in morphology and a change from expression of chondrocyte-specific genes to that of genes that are normally expressed in fibroblasts. Transfer of the monolayer chondrocyte culture to three-dimensional culture systems induces the cells to re-acquire a chondrocyte-specific phenotype and produce a cartilaginous-like tissue in vitro. We investigated mechanisms involved in the control of the de-differentiation and re-differentiation process in vitro. De-differentiated chondrocytes re-acquired their chondrocyte-specific phenotype when cultured on poly-(2-hydroxyethyl methacrylate) (polyHEMA) as assayed by morphology, reverse transcriptase PCR of chondrocyte-specific mRNA, Western-blot analysis and chondrocyte-specific promoter activity. Essentially, full recovery of the chondrocyte-specific phenotype was observed when cells that had been cultured for 4 weeks on plastic were transferred to culture on polyHEMA. However, after subsequent passages on plastic, the phenotype recovery was incomplete or did not occur. The activity of a gene reporter construct containing the promoter and enhancer from the human type-II collagen gene (COL2A1) was modulated by the culture conditions, so that its transcriptional activity was repressed in monolayer cultures and rescued to some extent when the cells were switched to polyHEMA cultures. The binding of Sry-type high-mobility-group box (SOX) transcription factors to the enhancer region was modulated by the culture conditions, as were the mRNA levels for SOX9. A transfected human type-II collagen reporter construct was activated in de-differentiated cells by ectopic expression of SOX transcription factors. These results underscore the overt change in phenotype that occurs when chondrocytes are cultured as monolayers on tissue-culture plastic substrata.

Indexed as

Gene Expression Regulation, DevelopmentalAnimalsBase SequenceCattleCell Culture TechniquesCell DifferentiationCells, CulturedChondrocytesCollagen Type IIDNA-Binding ProteinsEnhancer Elements, GeneticFetusGene Expression ProfilingHigh Mobility Group ProteinsHumansMolecular Sequence DataCollagen Type IIDNA-Binding ProteinsHigh Mobility Group ProteinsNuclear ProteinsSex-Determining Region Y ProteinSOX5 protein, humanSOX6 protein, humanSOX9 protein, humanSOX9 Transcription FactorSOXD Transcription FactorsSRY protein, humanTranscription Factors

Identifiers

PMID11716775
PMCPMC1222247

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.