ArticleThe Biochemical journal2001
myo-Inositol oxygenase: molecular cloning and expression of a unique enzyme that oxidizes myo-inositol and D-chiro-inositol.
Article in The Biochemical journal, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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Who cites it
39 citing papers in PubMed, 87 citations in OpenAlex.
- In silico characterization and molecular docking of the MIOX gene in Nile tilapia (Oreochromis niloticus).Fish physiology and biochemistry · 2026Article
- Myo-Inositol Oxygenase (MIOX): A Pivotal Regulator and Therapeutic Target in Multiple Diseases.Current issues in molecular biology · 2025Review
- Advances in basic biology of alfalfa (Horticulture research · 2025Article
- Cell factories for biosynthesis of D-glucaric acid: a fusion of static and dynamic strategies.World journal of microbiology & biotechnology · 2024Review
- L-Ascorbic acid metabolism and regulation in fruit crops.Plant physiology · 2023Article
- Inositol in Disease and Development: Roles of Catabolism viaInternational journal of molecular sciences · 2023Article
- Symbiotic microbes aid host adaptation by metabolizing a deterrent host pine carbohydrate d-pinitol in a beetle-fungus invasive complex.Science advances · 2022Article
- Potential of engineering the myo-inositol oxidation pathway to increase stress resilience in plants.Molecular biology reports · 2022Review
- Article
- New Insights into the Activities of D-Chiro-Inositol: A Narrative Review.Biomedicines · 2021Review
- Interrelationship of myo-inositol pathways with systemic metabolic conditions in two strains of high-performance laying hens during their productive life span.Scientific reports · 2021Article
- Cell-based and cell-free biocatalysis for the production of D-glucaric acid.Biotechnology for biofuels · 2020Review
- Kidney-based in vivo model for drug-induced nephrotoxicity testing.Scientific reports · 2020Article
- Myo-inositol: its metabolism and potential implications for poultry nutrition-a review.Poultry science · 2020Review
- Formation of Unstable and very Reactive Chemical Species Catalyzed by Metalloenzymes: A Mechanistic Overview.Molecules (Basel, Switzerland) · 2019Review
- HarnessingChemical science · 2019Article
- Metabolic engineering of Saccharomyces cerevisiae for efficient production of glucaric acid at high titer.Microbial cell factories · 2018Article
- Myo-inositol Oxygenase as a Novel Marker in the Diagnosis of Acute Kidney Injury.Journal of medical biochemistry · 2018Article
- Molecular cloning of the myo-inositol oxygenase gene from the kidney of baboons.Biomedical reports · 2017Article
- myo-Inositol Oxygenase Overexpression Accentuates Generation of Reactive Oxygen Species and Exacerbates Cellular Injury following High Glucose Ambience: A NEW MECHANISM RELEVANT TO THE PATHOGENESIS OF DIABETIC NEPHROPATHY.The Journal of biological chemistry · 2016Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
myo-Inositol oxygenase (MIOX) catalyses the first committed step in the only pathway of myo-inositol catabolism, which occurs predominantly in the kidney. The enzyme is a non-haem-iron enzyme that catalyses the ring cleavage of myo-inositol with the incorporation of a single atom of oxygen. A full-length cDNA was isolated from a pig kidney library with an open reading frame of 849 bp and a corresponding protein subunit molecular mass of 32.7 kDa. The cDNA was expressed in a bacterial pET expression system and an active recombinant MIOX was purified from bacterial lysates to electrophoretic homogeneity. The purified enzyme displayed the same catalytic properties as the native enzyme with K(m) and k(cat) values of 5.9 mM and 11 min(-1) respectively. The pI was estimated to be 4.5. Preincubation with 1 mM Fe(2+) and 2 mM cysteine was essential for the enzyme's activity. D-chiro-Inositol, a myo-inositol isomer, is a substrate for the recombinant MIOX with an estimated K(m) of 33.5 mM. Both myo-inositol and D-chiro-inositol have been implicated in the pathogenesis of diabetes. Thus an understanding of the regulation of MIOX expression clearly represents a potential window on the aetiology of diabetes as well as on the control of various intracellular phosphoinositides and key signalling pathways.
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