ArticleProtein science : a publication of the Protein Society2001
Catalytic and binding poly-reactivities shared by two unrelated proteins: The potential role of promiscuity in enzyme evolution.
Article in Protein science : a publication of the Protein Society, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
21 citing papers in PubMed.
- Serum Albumin in Health and Disease: Esterase, Antioxidant, Transporting and Signaling Properties.International journal of molecular sciences · 2021Review
- Promiscuous Ribozymes and Their Proposed Role in Prebiotic Evolution.Chemical reviews · 2020Review
- Modeling the Alkaline Hydrolysis of Diaryl Sulfate Diesters: A Mechanistic Study.The Journal of organic chemistry · 2020Article
- Evolutionary Relationships Between the Laccase Genes of Polyporales: Orthology-Based Classification of Laccase Isozymes and Functional Insight FromFrontiers in microbiology · 2019Article
- Isospecific adenine DNA methyltransferases show distinct preferences towards DNA substrates.Scientific reports · 2018Article
- Computational strategies for the design of new enzymatic functions.Archives of biochemistry and biophysics · 2015Review
- Are molecular alphabets universal enabling factors for the evolution of complex life?Origins of life and evolution of the biosphere : the journal of the International Society for the Study of the Origin of Life · 2013Article
- Ensemble perspective for catalytic promiscuity: calorimetric analysis of the active site conformational landscape of a detoxification enzyme.The Journal of biological chemistry · 2011Article
- Extending biochemical databases by metabolomic surveys.The Journal of biological chemistry · 2011Review
- Statistical analysis and molecular dynamics simulations of ambivalent α-helices.BMC bioinformatics · 2010Article
- Mutational effects and the evolution of new protein functions.Nature reviews. Genetics · 2010Review
- Evolution of hormone signaling in elasmobranchs by exploitation of promiscuous receptors.Molecular biology and evolution · 2008Article
- TRIM21 is an IgG receptor that is structurally, thermodynamically, and kinetically conserved.Proceedings of the National Academy of Sciences of the United States of America · 2008Article
- A structural model of latent evolutionary potentials underlying neutral networks in proteins.HFSP journal · 2007Article
- The role of peptide motifs in the evolution of a protein network.Nucleic acids research · 2007Article
- The evolution of catalytic efficiency and substrate promiscuity in human theta class 1-1 glutathione transferase.Journal of molecular biology · 2006Article
- Evolution of enzymatic activity in the tautomerase superfamily: mechanistic and structural consequences of the L8R mutation in 4-oxalocrotonate tautomerase.Biochemistry · 2006Article
- Control of stereoselectivity in an enzymatic reaction by backdoor access.Angewandte Chemie (International ed. in English) · 2006Article
- The specificity of cross-reactivity: promiscuous antibody binding involves specific hydrogen bonds rather than nonspecific hydrophobic stickiness.Protein science : a publication of the Protein Society · 2003Article
- SiteLight: binding-site prediction using phage display libraries.Protein science : a publication of the Protein Society · 2003Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
It is generally accepted that enzymes evolved via gene duplication of existing proteins. But duplicated genes can serve as a starting point for the evolution of a new function only if the protein they encode happens to exhibit some activity towards this new function. Although the importance of such catalytic promiscuity in enzyme evolution has been proposed, little is actually known regarding how common promiscuous catalytic activities are in proteins or their origins, magnitudes, and potential contribution to the survival of an organism. Here we describe a pattern of promiscuous activities in two completely unrelated proteins-serum albumins and a catalytic antibody (aldolase antibody 38C2). Despite considerable structural dissimilarities-in the shape of the cavities and the position of catalytic lysine residues-both active sites are able to catalyze the Kemp elimination, a model reaction for proton transfer from carbon. We also show that these different active sites can bind promiscuously an array of hydrophobic negatively charged ligands. We suggest that the basic active-site features of an apolar pocket and a lysine residue can act as a primitive active site allowing these promiscuous activities to take place. We also describe, by modelling product formation at different substrate concentrations, how promiscuous activities of this kind- inefficient and rudimentary as they are-can provide a considerable selective advantage and a starting point for the evolution of new functions.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.