ArticleNucleic acids research2001
A flexible light-directed DNA chip synthesis gated by deprotection using solution photogenerated acids.
Article in Nucleic acids research, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
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Who cites it
44 citing papers in PubMed, 144 citations in OpenAlex.
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- Recent progress in DNA data storage based on high-throughput DNA synthesis.Biomedical engineering letters · 2024Review
- POSoligo software for in vitro gene synthesis.Scientific reports · 2024Article
- Automated high-throughput DNA synthesis and assembly.Heliyon · 2024Review
- Miniaturized Control of Acidity in Multiplexed Microreactors.ACS omega · 2023Article
- Massively-multiplexed epitope mapping techniques for viral antigen discovery.Frontiers in immunology · 2023Review
- DNA synthesis technologies to close the gene writing gap.Nature reviews. Chemistry · 2023Review
- Biomolecule-Based Optical Metamaterials: Design and Applications.Biosensors · 2022Review
- CMOS electrochemical pH localizer-imager.Science advances · 2022Article
- Uncertainties in synthetic DNA-based data storage.Nucleic acids research · 2021Review
- Large-ScaleFrontiers in bioengineering and biotechnology · 2021Review
- Carbon-based archiving: current progress and future prospects of DNA-based data storage.GigaScience · 2019Review
- Cognizance of Molecular Methods for the Generation of Mutagenic Phage Display Antibody Libraries for Affinity Maturation.International journal of molecular sciences · 2019Review
- Peptide Arrays: Development and Application.Analytical chemistry · 2018Review
- Diagnostic microarray for 14 water and foodborne pathogens using a flatbed scanner.Journal of microbiological methods · 2017Article
- High-fidelity de novo synthesis of pathways using microchip-synthesized oligonucleotides and general molecular biology equipment.Scientific reports · 2017Article
- Modeling Hybridization Kinetics of Gene Probes in a DNA Biochip Using FEMLAB.Microarrays (Basel, Switzerland) · 2017Article
- Synthetic DNA Synthesis and Assembly: Putting the Synthetic in Synthetic Biology.Cold Spring Harbor perspectives in biology · 2017Review
- Next generation gene synthesis: From microarrays to genomes.Engineering in life sciences · 2017Review
Corrections and comments
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Oligonucleotide microarrays or oDNA chips are effective decoding and analytical tools for genomic sequences and are useful for a broad range of applications. Therefore, it is desirable to have synthesis methods of DNA chips that are highly flexible in sequence design and provide high quality and general adoptability. We report herein, DNA microarray synthesis based on a flexible biochip method. Our method simply uses photogenerated acid (PGA) in solution to trigger deprotection of the 5'-OH group in conventional nucleotide phosphoramidite monomers (i.e. PGA-gated deprotection), with the rest of the reactions in the synthesis cycle the same as those used for routine synthesis of oligonucleotides. The complete DNA chip synthesis process is accomplished on a regular DNA synthesizer that is coupled with a UV-VIS projection display unit for performing digital photolithography. Using this method, oDNA chips containing probes of newly discovered genes can be quickly and easily synthesized at high yields in a conventional laboratory setting. Furthermore, the PGA-gated chemistry should be applicable to microarray syntheses of a variety of combinatorial molecules, such as peptides and organic molecules.
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