ArticleNucleic acids research2001
Degradation of the unstable EP1 mRNA in Trypanosoma brucei involves initial destruction of the 3'-untranslated region.
Article in Nucleic acids research, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- Specialized RNA decay fine-tunes monogenic antigen expression in Trypanosoma brucei.Nature microbiology · 2026Article
- Differential regulatory role of AU-rich and GU-rich elements inFrontiers in microbiology · 2025Article
- Regulation of gene expression in trypanosomatids: living with polycistronic transcription.Open biology · 2019Article
- Transcriptome-wide analysis of trypanosome mRNA decay reveals complex degradation kinetics and suggests a role for co-transcriptional degradation in determining mRNA levels.Molecular microbiology · 2014Article
- The roles of 3'-exoribonucleases and the exosome in trypanosome mRNA degradation.RNA (New York, N.Y.) · 2013Article
- The role of the 5'-3' exoribonuclease XRNA in transcriptome-wide mRNA degradation.RNA (New York, N.Y.) · 2011Article
- The role of deadenylation in the degradation of unstable mRNAs in trypanosomes.Nucleic acids research · 2009Article
- Differential expression of a protease gene family in African trypanosomes.Molecular and biochemical parasitology · 2009Article
- Functionally related transcripts have common RNA motifs for specific RNA-binding proteins in trypanosomes.BMC molecular biology · 2008Article
- The RNA-binding protein TbDRBD3 regulates the stability of a specific subset of mRNAs in trypanosomes.Nucleic acids research · 2008Article
- Deadenylation-independent stage-specific mRNA degradation in Leishmania.Nucleic acids research · 2008Article
- A+U-rich instability elements differentially activate 5'-3' and 3'-5' mRNA decay.Molecular and cellular biology · 2007Article
- mRNA maturation by two-step trans-splicing/polyadenylation processing in trypanosomes.Proceedings of the National Academy of Sciences of the United States of America · 2007Article
- Roles of a Trypanosoma brucei 5'->3' exoribonuclease homolog in mRNA degradation.RNA (New York, N.Y.) · 2006Article
- Article
- Developmentally regulated instability of the GPI-PLC mRNA is dependent on a short-lived protein factor.Nucleic acids research · 2005Article
- A role for the exosome in the in vivo degradation of unstable mRNAs.RNA (New York, N.Y.) · 2003Article
- A negative regulatory element controls mRNA abundance of the Leishmania mexicana Paraflagellar rod gene PFR2.Eukaryotic cell · 2003Article
- Expression of the human RNA-binding protein HuR in Trypanosoma brucei increases the abundance of mRNAs containing AU-rich regulatory elements.Nucleic acids research · 2002Article
- Life without transcriptional control? From fly to man and back again.The EMBO journal · 2002Review
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2 authors.
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Abstract
Kinetoplastid protozoa regulate their gene expression primarily through control of mRNA degradation and translation. We describe here the degradation of three reporter mRNAs in Trypanosoma brucei. One mRNA had the 3'-untranslated region (3'-UTR) from the developmentally regulated EP1 mRNA, which is abundant in the procyclic (tsetse fly) form of the parasite but is almost undetectable in the bloodstream form. This untranslated region includes a 26 nt U-rich sequence that causes extreme RNA instability in the bloodstream form. The two other RNAs, which are not developmentally regulated, had either the actin 3'-UTR, or a version of the EP1 sequence lacking the 26 nt bloodstream-form instability element. All RNAs had poly(A) tails approximately 200 nt long, in both bloodstream and procyclic forms. Degradation of the two constitutively expressed mRNAs involved deadenylation and degradation by both 5'-->3' and 3'-->5' exonucleases. In contrast, in bloodstream forms, the 3'-end of the RNA bearing the bloodstream-form instability element disappeared very rapidly after transcription inhibition and partially deadenylated intermediates were not seen. The instability element may cause extremely rapid deadenylation, or it may be targeted by an endonuclease.
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