ArticleNucleic acids research2001
The abundance of sterile transcripts in Giardia lamblia.
Article in Nucleic acids research, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 59 citations in OpenAlex.
- RNA Sequencing Reveals Widespread Transcription of Natural Antisense RNAs inMicroorganisms · 2022Article
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- Recent advances in functional research in Giardia intestinalis.Advances in parasitology · 2020Review
- The giardial VPS35 retromer subunit is necessary for multimeric complex assembly and interaction with the vacuolar protein sorting receptor.Biochimica et biophysica acta · 2013Article
- Article
- Vacuolar protein sorting receptor in Giardia lamblia.PloS one · 2012Article
- A microRNA derived from an apparent canonical biogenesis pathway regulates variant surface protein gene expression in Giardia lamblia.RNA (New York, N.Y.) · 2011Article
- Identification and analysis of the RNA degrading complexes and machinery of Giardia lamblia using an in silico approach.BMC genomics · 2011Article
- Identification of transcriptional signals in Encephalitozoon cuniculi widespread among Microsporidia phylum: support for accurate structural genome annotation.BMC genomics · 2009Article
- High throughput genome-wide survey of small RNAs from the parasitic protists Giardia intestinalis and Trichomonas vaginalis.Genome biology and evolution · 2009Article
- snoRNA, a novel precursor of microRNA in Giardia lamblia.PLoS pathogens · 2008Article
- Noncoding RNA in development.Mammalian genome : official journal of the International Mammalian Genome Society · 2008Review
- Article
- Bidirectional transcription is an inherent feature of Giardia lamblia promoters and contributes to an abundance of sterile antisense transcripts throughout the genome.Nucleic acids research · 2007Article
- Evidence from bioinformatics, expression and inhibition studies of phosphoinositide-3 kinase signalling in Giardia intestinalis.BMC microbiology · 2006Article
- Do genes from the cholesterol synthesis pathway exist and express in Giardia intestinalis?Parasitology research · 2006Article
- A high frequency of overlapping gene expression in compacted eukaryotic genomes.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
- Small sense and antisense RNAs derived from a telomeric retroposon family in Giardia intestinalis.Eukaryotic cell · 2005Article
- RNA polymerase II synthesizes antisense RNA in Plasmodium falciparum.RNA (New York, N.Y.) · 2005Article
- Evolution of eukaryotic transcription: insights from the genome of Giardia lamblia.Genome research · 2004Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The protozoan parasite Giardia lamblia synthesizes a diverse and surprisingly abundant array of sterile transcripts unable to code for proteins. Random sampling of cDNAs from two evolutionarily divergent Giardia strains indicates that approximately 20% of cDNAs in the libraries represent polyadenylated sterile transcripts. RNase protection analysis and northern blot hybridization of three sterile transcript loci demonstrated that both the sterile transcript and a complementary mRNA were made in each case, further categorizing these sterile transcripts as antisense transcripts. Investigation of the genomic loci for these same three sterile antisense transcripts showed typical transcription units for the sense transcripts, but still failed to reveal a usable open reading frame for the sterile antisense transcripts. 5'-RACE mapped the transcription start site for one of the sterile antisense transcripts to an AT-rich region, as is typical for GIARDIA: It is unclear whether these sterile transcripts represent errors in transcription or whether they have regulatory functions within the cell, although preliminary investigations failed to reveal evidence for a role in developmental gene regulation. In either case, the presence of such a large pool of sterile antisense transcripts is dramatic evidence of the unusual molecular machinery of the early diverging protist G.lamblia.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.