ArticleNucleic acids research2001
Box C/D RNA guides for the ribose methylation of archaeal tRNAs. The tRNATrp intron guides the formation of two ribose-methylated nucleosides in the mature tRNATrp.
Article in Nucleic acids research, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 83 papers.
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83 citing papers in PubMed, 150 citations in OpenAlex.
- Small Nucleolar RNAs (snoRNAs) in Cancer: From Biogenesis to Clinical Potential.OncoTargets and therapy · 2026Review
- Archaeal RNA processing and regulation: expanding the functional landscape.Microbiology and molecular biology reviews : MMBR · 2025Review
- tRNA modifications: greasing the wheels of translation and beyond.RNA biology · 2025Review
- Archaea express circular isoforms of IS200/IS605-associated ωRNAs.Frontiers in microbiology · 2025Article
- Circular RNAs in Archaea.Advances in experimental medicine and biology · 2025Review
- Complicated target recognition by archaeal box C/D guide RNAs.Science China. Life sciences · 2024Article
- IGCNSDA: unraveling disease-associated snoRNAs with an interpretable graph convolutional network.Briefings in bioinformatics · 2024Article
- Variation of tRNA modifications with and without intron dependency.Frontiers in genetics · 2024Review
- A snoRNA-tRNA modification network governs codon-biased cellular states.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- C/D box snoRNA SNORD113-6 guides 2'-O-methylation and protects against site-specific fragmentation of tRNAMolecular therapy. Nucleic acids · 2022Article
- Biogenesis and Regulatory Roles of Circular RNAs.Annual review of cell and developmental biology · 2022Review
- The occurrence order and cross-talk of different tRNA modifications.Science China. Life sciences · 2021Review
- Emerging Data on the Diversity of Molecular Mechanisms Involving C/D snoRNAs.Non-coding RNA · 2021Review
- Conservation of Archaeal C/D Box sRNA-Guided RNA Modifications.Frontiers in microbiology · 2021Review
- Comparative patterns of modified nucleotides in individual tRNA species from a mesophilic and two thermophilic archaea.RNA (New York, N.Y.) · 2020Article
- Death by lipids: The role of small nucleolar RNAs in metabolic stress.The Journal of biological chemistry · 2020Review
- Splicing Endonuclease Is an Important Player in rRNA and tRNA Maturation in Archaea.Frontiers in microbiology · 2020Article
- Distinct Modified Nucleosides in tRNAJournal of bacteriology · 2019Article
- Impact of intron removal from tRNA genes on Saccharomyces cerevisiae.Nucleic acids research · 2019Article
- tRNA Modification Profiles and Codon-Decoding Strategies in Methanocaldococcus jannaschii.Journal of bacteriology · 2019Article
23 more citing papers are in PubMed but not listed here.
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Following a search of the Pyrococcus genomes for homologs of eukaryotic methylation guide small nucleolar RNAs, we have experimentally identified in Pyrococcus abyssi four novel box C/D small RNAs predicted to direct 2'-O-ribose methylations onto the first position of the anticodon in tRNALeu(CAA), tRNALeu(UAA), elongator tRNAMet and tRNATrp, respectively. Remarkably, one of them corresponds to the intron of its presumptive target, pre-tRNATrp. This intron is predicted to direct in cis two distinct ribose methylations within the unspliced tRNA precursor, not only onto the first position of the anticodon in the 5' exon but also onto position 39 (universal tRNA numbering) in the 3' exon. The two intramolecular RNA duplexes expected to direct methylation, which both span an exon-intron junction in pre-tRNATrp, are phylogenetically conserved in euryarchaeotes. We have experimentally confirmed the predicted guide function of the box C/D intron in halophile Haloferax volcanii by mutagenesis analysis, using an in vitro splicing/RNA modification assay in which the two cognate ribose methylations of pre-tRNATrp are faithfully reproduced. Euryarchaeal pre-tRNATrp should provide a unique system to further investigate the molecular mechanisms of RNA-guided ribose methylation and gain new insights into the origin and evolution of the complex family of archaeal and eukaryotic box C/D small RNAs.
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