ArticleMolecular and cellular biology2001
GAGA factor isoforms have distinct but overlapping functions in vivo.
Article in Molecular and cellular biology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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16 citing papers in PubMed, 34 citations in OpenAlex.
- Dual mitotic bookmarking by GAF and H3K27ac orchestrates differential propagation of cell fate memory in neural development.Nature communications · 2025Article
- Localization of the Drosophila pioneer factor GAF to subnuclear foci is driven by DNA binding and required to silence satellite repeat expression.Developmental cell · 2023Article
- Kinetic principles underlying pioneer function of GAGA transcription factor in live cells.Nature structural & molecular biology · 2022Article
- GAGA factor: a multifunctional pioneering chromatin protein.Cellular and molecular life sciences : CMLS · 2021Review
- GAGA Regulates Border Cell Migration inInternational journal of molecular sciences · 2020Article
- Different Evolutionary Strategies To Conserve Chromatin Boundary Function in the Bithorax Complex.Genetics · 2017Article
- GAGA factor, a positive regulator of global gene expression, modulates transcriptional pausing and organization of upstream nucleosomes.Epigenetics & chromatin · 2016Article
- Functional Requirements for Fab-7 Boundary Activity in the Bithorax Complex.Molecular and cellular biology · 2015Article
- Functional role of dimerization and CP190 interacting domains of CTCF protein in Drosophila melanogaster.BMC biology · 2015Article
- Drosophila GAGA factor polyglutamine domains exhibit prion-like behavior.BMC genomics · 2013Article
- Genetic basis for developmental homeostasis of germline stem cell niche number: a network of Tramtrack-Group nuclear BTB factors.PloS one · 2012Article
- A stage-specific factor confers Fab-7 boundary activity during early embryogenesis in Drosophila.Molecular and cellular biology · 2008Article
- General, negative feedback mechanism for regulation of Trithorax-like gene expression in vivo: new roles for GAGA factor in flies.Nucleic acids research · 2007Article
- The enhancer-blocking activity of the Fab-7 boundary from the Drosophila bithorax complex requires GAGA-factor-binding sites.Genetics · 2004Article
- batman Interacts with polycomb and trithorax group genes and encodes a BTB/POZ protein that is included in a complex containing GAGA factor.Molecular and cellular biology · 2003Article
- The GAGA protein is essential for dorsal appendage formation during Drosophila melanogaster oogenesis.Doklady. Biochemistry and biophysicsArticle
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2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The Drosophila melanogaster GAGA factor (encoded by the Trithorax-like [Trl] gene) is required for correct chromatin architecture at diverse chromosomal sites. The Trl gene encodes two alternatively spliced isoforms of the GAGA factor (GAGA-519 and GAGA-581) that are identical except for the length and sequence of the C-terminal glutamine-rich (Q) domain. In vitro and tissue culture experiments failed to find any functional difference between the two isoforms. We made a set of transgenes that constitutively express cDNAs coding for either of the isoforms with the goal of elucidating their roles in vivo. Phenotypic analysis of the transgenes in Trl mutant background led us to the conclusion that GAGA-519 and GAGA-581 perform different, albeit largely overlapping, functions. We also expressed a fusion protein with LacZ disrupting the Q domain of GAGA-519. This LacZ fusion protein compensated for the loss of wild-type GAGA factor to a surprisingly large extent. This suggests that the Q domain either is not required for the essential functions performed by the GAGA protein or is exclusively used for tetramer formation. These results are inconsistent with a major role of the Q domain in chromatin remodeling or transcriptional activation. We also found that GAGA-LacZ was able to associate with sites not normally occupied by the GAGA factor, pointing to a role of the Q domain in binding site choice in vivo.
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