ArticleMolecular and cellular biology2001
Saturation mutagenesis of 5S rRNA in Saccharomyces cerevisiae.
Article in Molecular and cellular biology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 49 citations in OpenAlex.
- Sordarin bound eEF2 unlocks spontaneous forward and reverse translocation on CrPV IRES.Nucleic acids research · 2023Article
- Cryo-electron microscopy visualization of a large insertion in the 5S ribosomal RNA of the extremely halophilic archaeon Halococcus morrhuae.FEBS open bio · 2020Article
- Ribosome engineering reveals the importance of 5S rRNA autonomy for ribosome assembly.Nature communications · 2020Article
- The L1 stalk is required for efficient export of nascent large ribosomal subunits in yeast.RNA (New York, N.Y.) · 2019Article
- Multi-protein bridging factor 1(Mbf1), Rps3 and Asc1 prevent stalled ribosomes from frameshifting.eLife · 2018Article
- Ribosome biogenesis in african trypanosomes requires conserved and trypanosome-specific factors.Eukaryotic cell · 2014Article
- Mutations in the chromodomain-like insertion of translation elongation factor 3 compromise protein synthesis through reduced ATPase activity.The Journal of biological chemistry · 2014Article
- Review
- RNA SHAPE analysis in living cells.Nature chemical biology · 2013Article
- Biological significance of 5S rRNA import into human mitochondria: role of ribosomal protein MRP-L18.Genes & development · 2011Article
- Mitochondrial enzyme rhodanese is essential for 5 S ribosomal RNA import into human mitochondria.The Journal of biological chemistry · 2010Article
- Correlation of RNA secondary structure statistics with thermodynamic stability and applications to folding.Journal of molecular biology · 2009Article
- Bud23 methylates G1575 of 18S rRNA and is required for efficient nuclear export of pre-40S subunits.Molecular and cellular biology · 2008Article
- Two distinct structural elements of 5S rRNA are needed for its import into human mitochondria.RNA (New York, N.Y.) · 2008Article
- rRNA mutants in the yeast peptidyltransferase center reveal allosteric information networks and mechanisms of drug resistance.Nucleic acids research · 2008Article
- Two trypanosome-specific proteins are essential factors for 5S rRNA abundance and ribosomal assembly in Trypanosoma brucei.Eukaryotic cell · 2007Article
- Changes in the conformation of 5S rRNA cause alterations in principal functions of the ribosomal nanomachine.Nucleic acids research · 2007Article
- An arc of unpaired "hinge bases" facilitates information exchange among functional centers of the ribosome.Molecular and cellular biology · 2006Article
- Structural and functional analysis of 5S rRNA in Saccharomyces cerevisiae.Molecular genetics and genomics : MGG · 2005Article
- 5S rRNA: Structure and Function from Head to Toe.International journal of biomedical science : IJBS · 2005Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 3 countries.
Funding
Abstract
rRNAs are the central players in the reactions catalyzed by ribosomes, and the individual rRNAs are actively involved in different ribosome functions. Our previous demonstration that yeast 5S rRNA mutants (called mof9) can impact translational reading frame maintenance showed an unexpected function for this ubiquitous biomolecule. At the time, however, the highly repetitive nature of the genes encoding rRNAs precluded more detailed genetic and molecular analyses. A new genetic system allows all 5S rRNAs in the cell to be transcribed from a small, easily manipulated plasmid. The system is also amenable for the study of the other rRNAs, and provides an ideal genetic platform for detailed structural and functional studies. Saturation mutagenesis reveals regions of 5S rRNA that are required for cell viability, translational accuracy, and virus propagation. Unexpectedly, very few lethal alleles were identified, demonstrating the resilience of this molecule. Superimposition of genetic phenotypes on a physical map of 5S rRNA reveals the existence of phenotypic clusters of mutants, suggesting that specific regions of 5S rRNA are important for specific functions. Mapping these mutants onto the Haloarcula marismortui large subunit reveals that these clusters occur at important points of physical interaction between 5S rRNA and the different functional centers of the ribosome. Our analyses lead us to propose that one of the major functions of 5S rRNA may be to enhance translational fidelity by acting as a physical transducer of information between all of the different functional centers of the ribosome.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.