ArticleEMBO reports2001
Evidence for coupling of membrane targeting and function of the signal recognition particle (SRP) receptor FtsY.
Article in EMBO reports, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 45 citations in OpenAlex.
- Article
- The Archaeal Signal Recognition Particle: Present Understanding and Future Perspective.Current microbiology · 2017Review
- Small Regulatory RNAs of Rickettsia conorii.Scientific reports · 2016Article
- New approaches to the problem of generating coherent, reproducible phenotypes.Theory in biosciences = Theorie in den Biowissenschaften · 2014Article
- Lipids trigger a conformational switch that regulates signal recognition particle (SRP)-mediated protein targeting.The Journal of biological chemistry · 2011Article
- Article
- Depletion of the signal recognition particle receptor inactivates ribosomes in Escherichia coli.Journal of bacteriology · 2009Article
- Article
- Conformational changes in the GTPase modules of the signal reception particle and its receptor drive initiation of protein translocation.The Journal of cell biology · 2007Article
- X-ray structure of the T. aquaticus FtsY:GDP complex suggests functional roles for the C-terminal helix of the SRP GTPases.Proteins · 2007Article
- Membrane binding of the bacterial signal recognition particle receptor involves two distinct binding sites.The Journal of cell biology · 2006Article
- Localization of translocation complex components in Bacillus subtilis: enrichment of the signal recognition particle receptor at early sporulation septa.Journal of bacteriology · 2005Article
- Target-directed proteolysis at the ribosome.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
- The core Escherichia coli signal recognition particle receptor contains only the N and G domains of FtsY.Journal of bacteriology · 2004Article
- Secretion of LamB-LacZ by the signal recognition particle pathway of Escherichia coli.Journal of bacteriology · 2003Article
- The ribosome and YidC. New insights into the biogenesis of Escherichia coli inner membrane proteins.EMBO reports · 2003Review
- Accumulation of endoplasmic membranes and novel membrane-bound ribosome-signal recognition particle receptor complexes in Escherichia coli.The Journal of cell biology · 2002Article
Corrections and comments
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Authors and funding
12 authors at 6 institutions in 5 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Recent studies have indicated that FtsY, the signal recognition particle receptor of Escherichia coli, plays a central role in membrane protein biogenesis. For proper function, FtsY must be targeted to the membrane, but its membrane-targeting pathway is unknown. We investigated the relationship between targeting and function of FtsY in vivo, by separating its catalytic domain (NG) from its putative targeting domain (A) by three means: expression of split ftsY, insertion of various spacers between A and NG, and separation of A and NG by in vivo proteolysis. Proteolytic separation of A and NG does not abolish function, whereas separation by long linkers or expression of split ftsY is detrimental. We propose that proteolytic cleavage of FtsY occurs after completion of co-translational targeting and assembly of NG. In contrast, separation by other means may interrupt proper synchronization of co-translational targeting and membrane assembly of NG. The co-translational interaction of FtsY with the membrane was confirmed by in vitro experiments.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.