ArticleThe Journal of physiology2001
Alternative splicing determines sensitivity of murine calcium-activated potassium channels to glucocorticoids.
Article in The Journal of physiology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 56 citations in OpenAlex.
- Molecular Derangements and the Diagnosis of ACTH-Dependent Cushing's Syndrome.Endocrine reviews · 2022Review
- Role of glucocorticoid negative feedback in the regulation of HPA axis pulsatility.Stress (Amsterdam, Netherlands) · 2018Review
- Glucocorticoids Inhibit CRH/AVP-Evoked Bursting Activity of Male Murine Anterior Pituitary Corticotrophs.Endocrinology · 2016Article
- Unoprostone activation of BK (KCa1.1) channel splice variants.Biochimica et biophysica acta · 2015Article
- Large conductance Ca²⁺-activated K⁺ (BK) channels promote secretagogue-induced transition from spiking to bursting in murine anterior pituitary corticotrophs.The Journal of physiology · 2015Article
- Large conductance voltage- and Ca2+-gated potassium (BK) channel β4 subunit influences sensitivity and tolerance to alcohol by altering its response to kinases.The Journal of biological chemistry · 2014Article
- Article
- Splicing of the rSlo gene affects the molecular composition and drug response of Ca2+-activated K+ channels in skeletal muscle.PloS one · 2012Article
- Corticosterone pretreatment suppresses stress-induced hypothalamic-pituitary-adrenal axis activity via multiple actions that vary with time, site of action, and de novo protein synthesis.The Journal of endocrinology · 2011Article
- Upregulation of STREX splice variant of the large conductance Ca2+-activated potassium (BK) channel in a rat model of mesial temporal lobe epilepsy.Neuroscience research · 2011Article
- Intron retention facilitates splice variant diversity in calcium-activated big potassium channel populations.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- Ion channels and signaling in the pituitary gland.Endocrine reviews · 2010Review
- Expression of BK-type calcium-activated potassium channel splice variants during chick cochlear development.The Journal of comparative neurology · 2010Article
- Adrenaline-induced colonic K+ secretion is mediated by KCa1.1 (BK) channels.The Journal of physiology · 2010Article
- Colonic potassium handling.Pflugers Archiv : European journal of physiology · 2010Review
- Protein-protein interaction between cPLA2 and splice variants of alpha-subunit of BK channels.American journal of physiology. Cell physiology · 2010Article
- Alpha5beta1 integrin engagement increases large conductance, Ca2+-activated K+ channel current and Ca2+ sensitivity through c-src-mediated channel phosphorylation.The Journal of biological chemistry · 2010Article
- Mechanoregulation of BK channel activity in the mammalian cortical collecting duct: role of protein kinases A and C.American journal of physiology. Renal physiology · 2009Article
- Heterogeneity in function of small artery smooth muscle BKCa: involvement of the beta1-subunit.The Journal of physiology · 2009Article
- Control of alternative pre-mRNA splicing by Ca(++) signals.Biochimica et biophysica acta · 2008Review
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
1. Large-conductance Ca(2+)- and voltage-activated potassium (BK) channels are important regulators of cellular excitability. Here, we present a patch-clamp electrophysiological analysis of splice-variant-specific regulation by the synthetic glucocorticoid dexamethasone (DEX) of BK channels consisting of cloned STREX or ZERO alpha-subunit variants expressed in human embryonic kidney (HEK 293) cells. 2. STREX channels in isolated membrane patches were inhibited by protein kinase A (PKA) and this was blocked on pre-treatment of intact cells with DEX (100 nM) for 2 h. 3. The effect of DEX required the synthesis of new mRNA and protein. Furthermore, it required protein phosphatase 2A (PP2A)-like activity intimately associated with the channels, as it was blocked by 10 nM okadaic acid but not by the specific protein phosphatase-1 inhibitor peptide PPI-2. 4. ZERO variant channels that lack the STREX insert were activated by PKA but were not influenced by DEX. ZERO channels containing a mutant STREX domain (S4(STREX)A) were also activated by PKA. Importantly, DEX blocked PKA activation of S4(STREX)A channels in a PP2A-dependent manner. 5. Taken together, the STREX domain is crucial for glucocorticoid regulation of BK channels through a PP2A-type enzyme. Moreover, glucocorticoids appear to induce a generic set of proteins in different types of cells, the actions of which depend on the expression of cell-specific targets.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.