ArticleJournal of bioenergetics and biomembranes2001
Palmitic and stearic acids bind Ca2+ with high affinity and form nonspecific channels in black-lipid membranes. Possible relation to Ca2+-activated mitochondrial pores.
Article in Journal of bioenergetics and biomembranes, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 55 citations in OpenAlex.
- The Short-Term Opening of Cyclosporin A-Independent Palmitate/SrMembranes · 2022Article
- Review
- Mitochondrial Uncoupling Proteins: Subtle Regulators of Cellular Redox Signaling.Antioxidants & redox signaling · 2018Review
- Inorganic polyphosphate (polyP) as an activator and structural component of the mitochondrial permeability transition pore.Biochemical Society transactions · 2016Review
- Effect of surface-potential modulators on the opening of lipid pores in liposomal and mitochondrial inner membranes induced by palmitate and calcium ions.Biochimica et biophysica acta · 2015Article
- The Mitochondrial Permeability Transition Pore: Channel Formation by F-ATP Synthase, Integration in Signal Transduction, and Role in Pathophysiology.Physiological reviews · 2015Review
- A permeability transition in liver mitochondria and liposomes induced by α,ω-dioic acids and Ca(2+).European biophysics journal : EBJ · 2014Article
- Oxidative phosphorylation and ion transport in the mitochondria of two strains of rats varying in their resistance to stress and hypoxia.Molecular and cellular biochemistry · 2013Article
- The mitochondrial permeability transition pore: a mystery solved?Frontiers in physiology · 2013Article
- Palmitic acid induces the opening of a Ca2+-dependent pore in the plasma membrane of red blood cells: the possible role of the pore in erythrocyte lysis.The Journal of membrane biology · 2010Article
- Physiological aspects of the mitochondrial cyclosporin A-insensitive palmitate/Ca2+-induced pore: tissue specificity, age profile and dependence on the animal's adaptation to hypoxia.Journal of bioenergetics and biomembranes · 2009Article
- The role of fatty acid unsaturation in minimizing biophysical changes on the structure and local effects of bilayer membranes.Biochimica et biophysica acta · 2009Article
- Induction of a non-specific permeability transition in mitochondria from Yarrowia lipolytica and Dipodascus (Endomyces) magnusii yeasts.Journal of bioenergetics and biomembranes · 2009Article
- Mitochondrial Ca2+ cycle mediated by the palmitate-activated cyclosporin A-insensitive pore.Journal of bioenergetics and biomembranes · 2007Article
- Ca2+-induced phase separation in the membrane of palmitate-containing liposomes and its possible relation to membrane permeabilization.The Journal of membrane biology · 2007Article
- On the mechanism of palmitic acid-induced apoptosis: the role of a pore induced by palmitic acid and Ca2+ in mitochondria.Journal of bioenergetics and biomembranes · 2006Article
- A large, voltage-dependent channel, isolated from mitochondria by water-free chloroform extraction.Biophysical journal · 2005Article
- Formation of palmitic acid/Ca2+ complexes in the mitochondrial membrane: a possible role in the cyclosporin-insensitive permeability transition.Journal of bioenergetics and biomembranes · 2004Article
- Fatty acids enhance membrane permeabilization by pro-apoptotic Bax.The Biochemical journal · 2004Article
Corrections and comments
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Authors and funding
10 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A mitochondrial hydrophobic component that forms Ca2+-induced nonspecific ion channels in black-lipid membranes (Mironova et al., 1997) has been purified and its nature elucidated. It consists of long-chain saturated fatty acids--mainly palmitic and stearic. These fatty acids, similar to the mitochondrial hydrophobic component, bind Ca2+ with high affinity in comparison with unsaturated fatty acids, saturated fatty acids with shorter aliphatic chains, phospholipids, and other lipids. Ca2+-binding is inhibited by Mg2+ but not by K+. For palmitic acid, the Kd for Ca2+ was 5 microM at pH 8.5 and 15 microM at pH 7.5, with the Bmax of 0.48 +/- 0.08 mmol/g. This corresponds to one Ca2+ ion for eight palmitic acid molecules. The data of IR spectroscopy confirm that Ca2+ does not form ionic bonds with palmitic and stearic acids under hydrophobic conditions. It has been found that in the presence of Ca2+, palmitic and stearic acids, but not unsaturated FFA induce a nonspecific permeability in black-lipid membranes. Addition of Ca2+ in order to induce the permeability transition, increases the extractable amount of palmitic and stearic acids, the effect being prevented by a phospholipase A2 inhibitor. The possible involvement of palmitic and stearic acids in the mitochondrial nonspecific permeability is discussed.
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