ArticleOncogene2001
Silencing of HTR1B and reduced expression of EDN1 in human lung cancers, revealed by methylation-sensitive representational difference analysis.
Article in Oncogene, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 56 citations in OpenAlex.
- Review
- Serotonin signalling in cancer: Emerging mechanisms and therapeutic opportunities.Clinical and translational medicine · 2024Review
- Hallmark guided identification and characterization of a novel immune-relevant signature for prognostication of recurrence in stage I-III lung adenocarcinoma.Genes & diseases · 2023Article
- Transcriptional profiling revealed the anti-proliferative effect of MFN2 deficiency and identified risk factors in lung adenocarcinoma.Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine · 2016Article
- Bioinformatic analyses revealed underlying biological functions correlated with oxaliplatin responsiveness.Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine · 2016Article
- Discovery of prognostic biomarkers for predicting lung cancer metastasis using microarray and survival data.BMC bioinformatics · 2015Article
- Serotonin 1B Receptor Gene (HTR1B) Methylation as a Risk Factor for Callous-Unemotional Traits in Antisocial Boys.PloS one · 2015Article
- Discovery and validation of methylation markers for endometrial cancer.International journal of cancer · 2014Article
- Disruption of the expression and function of microRNAs in lung cancer as a result of epigenetic changes.Frontiers in genetics · 2013Review
- Implications of Dll4-Notch signaling activation in primary glioblastoma multiforme.Neuro-oncology · 2013Article
- Methylation profiling identifies 2 groups of gliomas according to their tumorigenesis.Neuro-oncology · 2011Article
- Comprehensive profiling of DNA methylation in colorectal cancer reveals subgroups with distinct clinicopathological and molecular features.BMC cancer · 2010Article
- Integrating the multiple dimensions of genomic and epigenomic landscapes of cancer.Cancer metastasis reviews · 2010Review
- Depletion of serotonin and selective inhibition of 2B receptor suppressed tumor angiogenesis by inhibiting endothelial nitric oxide synthase and extracellular signal-regulated kinase 1/2 phosphorylation.Neoplasia (New York, N.Y.) · 2009Article
- DNA methylation-based biomarkers for early detection of non-small cell lung cancer: an update.Molecular cancer · 2008Review
- Isolation and bioinformatics analysis of differentially methylated genomic fragments in human gastric cancer.World journal of gastroenterology · 2008Article
- A second-generation profiling system for quantitative methylation analysis of multiple gene promoters: application to lung cancer.Oncogene · 2007Article
- Frequent reduced expression of alpha-1B-adrenergic receptor caused by aberrant promoter methylation in gastric cancers.British journal of cancer · 2007Article
- Endothelin-1 inhibits apoptosis in prostate cancer.Neoplasia (New York, N.Y.) · 2005Article
- Identification of 27 5' CpG islands aberrantly methylated and 13 genes silenced in human pancreatic cancers.Oncogene · 2004Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Aberrantly hypermethylated genes in human lung cancers were searched for by a genome scanning technique, methylation-sensitive-representational difference analysis (MS-RDA). A total of 59 DNA fragments were isolated as those methylated more heavily in either/both of two lung squamous cell carcinoma cell lines, EBC-1 and LK-2, than in a primary culture of normal human bronchial epithelium, NHBE. Thirty-four DNA fragments, whose hypermethylation was confirmed in primary squamous cell carcinomas, were sequenced. By database searches, 17 of them were shown to be located within 2 kb of putative CpG islands, and five of the 17 DNA fragments had transcribed regions of known genes in their vicinities. By RT-PCR of the five genes in the carcinoma cell lines and NHBE, decreased expression of HTR1B (5-hydroxytryptamine receptor 1B) and EDN1 (endothelin-1) was observed. Sequencing after bisulfite modification showed that the CpG island in the promoter region of HTR1B was hypermethylated, while that of EDN1 was not. Demethylation and re-expression of HTR1B were observed after treatment of LK-2 cells with 5-aza-2'-deoxycytidine. In primary lung cancers, decreased mRNA expression of HTR1B was observed in 11 of 20 cases, and that of EDN1 was in 16 of 20 cases. Immunohistochemical analysis of endothelin-1 confirmed that its immunoreactivity was reduced in squamous cell carcinoma cells compared with that in normal bronchial epithelial cells. Considering that endothelin-1 induces apoptosis in melanoma cells and that silencing of endothelin receptor B is observed in prostate cancers, its reduced expression was speculated to confer a growth advantage to lung cancer cells. MS-RDA was shown to isolate DNA fragments that are hypermethylated and silenced, such as HTR1B, and those whose expressions are altered and the methylation statuses outside the promoter region are altered, such as EDN1.
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