ArticleAntimicrobial agents and chemotherapy2001
Characterization of OXA-29 from Legionella (Fluoribacter) gormanii: molecular class D beta-lactamase with unusual properties.
Article in Antimicrobial agents and chemotherapy, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 36 citations in OpenAlex.
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- Extensive epigenetic modification with large-scale chromosomal and plasmid recombination characterise the Legionella longbeachae serogroup 1 genome.Scientific reports · 2022Article
- Genomic characterization and assessment of pathogenic potential ofFrontiers in microbiology · 2022Article
- Closed Genome and Plasmid Sequences of Legionella pneumophila AW-13-4, Isolated from a Hot Water Loop System of a Large Occupational Building.Microbiology resource announcements · 2021Article
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- Detection ofMicroorganisms · 2018Article
- Purification and characterization of a new β-lactamase OXA-205 from Pseudomonas aeruginosa.Annals of clinical microbiology and antimicrobials · 2015Article
- Class D β-lactamases: a reappraisal after five decades.Accounts of chemical research · 2013Review
- Reclamation of ampicillin sensitivity for the genetic manipulation of Legionella pneumophila.Applied and environmental microbiology · 2012Article
- Diversity, epidemiology, and genetics of class D beta-lactamases.Antimicrobial agents and chemotherapy · 2010Review
- AmpC beta-lactamases.Clinical microbiology reviews · 2009Review
- Saturation mutagenesis of Asn152 reveals a substrate selectivity switch in P99 cephalosporinase.Protein science : a publication of the Protein Society · 2007Article
- Genetic and biochemical characterization of FUS-1 (OXA-85), a narrow-spectrum class D beta-lactamase from Fusobacterium nucleatum subsp. polymorphum.Antimicrobial agents and chemotherapy · 2006Article
- OXA-46, a new class D beta-lactamase of narrow substrate specificity encoded by a blaVIM-1-containing integron from a Pseudomonas aeruginosa clinical isolate.Antimicrobial agents and chemotherapy · 2005Article
- Biochemical characterization of the naturally occurring oxacillinase OXA-50 of Pseudomonas aeruginosa.Antimicrobial agents and chemotherapy · 2004Article
- Molecular and biochemical characterization of OXA-45, an extended-spectrum class 2d' beta-lactamase in Pseudomonas aeruginosa.Antimicrobial agents and chemotherapy · 2003Article
- EBR-1, a novel Ambler subclass B1 beta-lactamase from Empedobacter brevis.Antimicrobial agents and chemotherapy · 2002Article
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Authors and funding
9 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A class D beta-lactamase determinant was isolated from the genome of Legionella (Fluoribacter) gormanii ATCC 33297(T). The enzyme, named OXA-29, is quite divergent from other class D beta-lactamases, being more similar (33 to 43% amino acid identity) to those of groups III (OXA-1) and IV (OXA-9, OXA-12, OXA-18, and OXA-22) than to other class D enzymes (21 to 24% sequence identity). Phylogenetic analysis confirmed the closer ancestry of OXA-29 with members of the former groups. The OXA-29 enzyme was purified from an Escherichia coli strain overexpressing the gene via a T7-based expression system by a single ion-exchange chromatography step on S-Sepharose. The mature enzyme consists of a 28.5-kDa polypeptide and exhibits an isoelectric pH of >9. Analysis of the kinetic parameters of OXA-29 revealed efficient activity (k(cat)/K(m) ratios of >10(5) M(-1) x s(-1)) for several penam compounds (oxacillin, methicillin, penicillin G, ampicillin, carbenicillin, and piperacillin) and also for cefazolin and nitrocefin. Oxyimino cephalosporins and aztreonam were also hydrolyzed, although less efficiently (k(cat)/K(m) ratios of around 10(3) M(-1) x s(-1)). Carbapenems were neither hydrolyzed nor inhibitory. OXA-29 was inhibited by BRL 42715 (50% inhibitory concentration [IC(50)], 0.44 microM) and by tazobactam (IC(50), 3.2 microM), but not by clavulanate. It was also unusually resistant to chloride ions (IC(50), >100 mM). Unlike OXA-10, OXA-29 was apparently found as a dimer both in diluted solutions and in the presence of EDTA. Its activity was either unaffected or inhibited by divalent cations. OXA-29 is a new class D beta-lactamase that exhibits some unusual properties likely reflecting original structural and mechanistic features.
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