ArticleTransgenic research2001
Rapid in vivo functional analysis of transgenes in mice using whole body imaging of luciferase expression.
Article in Transgenic research, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
30 citing papers in PubMed, 117 citations in OpenAlex.
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- Molecular Imaging with Reporter Genes: Has Its Promise Been Delivered?Journal of nuclear medicine : official publication, Society of Nuclear Medicine · 2019Review
- APS Presidential Plenary 2019: the way of science: serendipity and the illusion of linearity.Pediatric research · 2019Article
- Glucuronidation of D-Luciferin In Vitro: Isoform Selectivity and Kinetics Characterization.European journal of drug metabolism and pharmacokinetics · 2019Article
- Noninvasive Imaging of Stored Red Blood Cell-Transfusion Aggravating Sepsis-Induced Liver Injury Associated with Increased Activation of M1-Polarized Kupffer Cells.Shock (Augusta, Ga.) · 2017Article
- MiR-196a regulates heme oxygenase-1 by silencing Bach1 in the neonatal mouse lung.American journal of physiology. Lung cellular and molecular physiology · 2016Article
- In vivo imaging of clock gene expression in multiple tissues of freely moving mice.Nature communications · 2016Article
- In vivo bioimaging with tissue-specific transcription factor activated luciferase reporters.Scientific reports · 2015Article
- Dynamic imaging of pancreatic nuclear factor κB (NF-κB) activation in live mice using adeno-associated virus (AAV) infusion and bioluminescence.The Journal of biological chemistry · 2015Article
- Opening up the optical imaging window using nano-luciferin.Pharmaceutical research · 2014Article
- William A Silverman lecture.Journal of perinatology : official journal of the California Perinatal Association · 2014Article
- Establishment of stable reporter expression for in vivo imaging of nuclear factor-κB activation in mouse liver.Theranostics · 2013Article
- Lung fluorescence imaging to evaluate tumor metastasis induced by AQP5 expression in murine model.Medical oncology (Northwood, London, England) · 2012Article
- Article
- Enhanced in vivo bioluminescence imaging using liposomal luciferin delivery system.Journal of controlled release : official journal of the Controlled Release Society · 2010Article
- Molecular mechanism and functional consequences of lansoprazole-mediated heme oxygenase-1 induction.World journal of gastroenterology · 2009Article
- Bioluminescence imaging of heme oxygenase-1 upregulation in the Gua Sha procedure.Journal of visualized experiments : JoVE · 2009Article
- Bach1 modulates heme oxygenase-1 expression in the neonatal mouse lung.Pediatric research · 2009Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 4 institutions in 1 country.
Funding
Abstract
The use of transgenic animals in biomedical research is increasing rapidly and may be the best means of determining gene function. Generating transgenic animals typically requires time-consuming screening processes, and gene function is assessed by an array of difficult phenotypic and biochemical assays performed ex vivo. To address the unmet need in transgenic research for functional assays performed with ease in living animals, we demonstrate here that in vivo detection of luciferase enzyme as a transcriptional reporter facilitates rapid screening for both the presence and function of transgenes in intact living mice. Using this approach we identified three bioluminescent transgenic founders where the transgene consisted of the heme oxygenase promoter fused to the modified coding sequence of the luciferase gene. These founders were identified from 183 pups and confirmed by PCR analysis. Identification of HO-1-luc homozygotes from back- crossed F2 littermates was then accelerated by in vivo imaging. In another transgenic mouse line, where the transgene was comprised of the bone morphogenic-4 (BMP4) promoter fused to the modified luciferase gene, we were able to identify transgenic animals and in each line we were able to visualize patterns of expression in living animals over time. The light production from these transgenic mice indicated that the desired DNA fragment was functional and different expression profiles apparent at different ages and after gene induction.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.