ArticleTransgenic research2001
Selection and orientation of adjacent genes influences DAM-mediated male sterility in transformed maize.
Article in Transgenic research, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 31 citations in OpenAlex.
- Highly efficient generation of bacterial leaf blight-resistant and transgene-free rice using a genome editing and multiplexed selection system.BMC plant biology · 2021Article
- Uniform Expression and Relatively Small Position Effects Characterize Sister Transformants in Maize and Soybean.Frontiers in plant science · 2019Article
- Construction of a multicontrol sterility system for a maize male-sterile line and hybrid seed production based on the ZmMs7 gene encoding a PHD-finger transcription factor.Plant biotechnology journal · 2018Article
- A single point mutation in Ms44 results in dominant male sterility and improves nitrogen use efficiency in maize.Plant biotechnology journal · 2017Article
- Targeted mutagenesis of a conserved anther-expressed P450 gene confers male sterility in monocots.Plant biotechnology journal · 2017Article
- Overexpression of ARGOS Genes Modifies Plant Sensitivity to Ethylene, Leading to Improved Drought Tolerance in Both Arabidopsis and Maize.Plant physiology · 2015Article
- Novel Strategies for Genomic Manipulation of Trichoderma reesei with the Purpose of Strain Engineering.Applied and environmental microbiology · 2015Article
- Transcriptional silencing of heterologous anther promoters in maize: a genetic method to replace detasseling for seed production.Plant reproduction · 2014Article
- Maize ARGOS1 (ZAR1) transgenic alleles increase hybrid maize yield.Journal of experimental botany · 2014Article
- Wolbachia prophage DNA adenine methyltransferase genes in different Drosophila-Wolbachia associations.PloS one · 2011Article
- The FT-like ZCN8 Gene Functions as a Floral Activator and Is Involved in Photoperiod Sensitivity in Maize.The Plant cell · 2011Article
- Concerted modification of flowering time and inflorescence architecture by ectopic expression of TFL1-like genes in maize.Plant physiology · 2010Article
- Cell Number Regulator1 affects plant and organ size in maize: implications for crop yield enhancement and heterosis.The Plant cell · 2010Article
- A dominant mutation in mediator of paramutation2, one of three second-largest subunits of a plant-specific RNA polymerase, disrupts multiple siRNA silencing processes.PLoS genetics · 2009Article
- Involvement of the MADS-box gene ZMM4 in floral induction and inflorescence development in maize.Plant physiology · 2008Article
- Health considerations regarding horizontal transfer of microbial transgenes present in genetically modified crops.Journal of biomedicine & biotechnology · 2005Article
- A chimeric ecdysone receptor facilitates methoxyfenozide-dependent restoration of male fertility in ms45 maize.Transgenic research · 2002Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Anther-targeted expression of E. coli DNA (Adenosine-N6-)-Methyltransferase (DAM) in maize was tested as a means to produce male-sterile plants. A high frequency of male-sterile plants with reduced anther size was observed when DAM was regulated by the maize anther-specific promoter 5126 (5126:DAM) and placed upstream of the herbicide resistance gene, pat, regulated by the cauliflower mosaic virus (CaMV) 35S promoter (35S:PAT). In contrast, placement of 5126:DAM upstream of a pat gene regulated by either the maize ubiquitin (UBI:PAT) or rice actin (rACTIN:PAT) promoters resulted in male-fertile plants. Based on these observed differences, DAM-mediated sterility was used as a phenotypic marker to assess the contribution of factors affecting gene expression such as orientation of the transcription units, choice of regulatory sequences mediating expression of adjacent genes, and effects of varying the anther-specific promoter regulating DAM. Constructs that place a portion of the CaMV 35S promoter, including the native AS-1 sequences, between 5126:DAM and UBI:PAT yielded a high frequency of male-sterile plants with reduced anther size. Significant differences in the frequency of male-sterile events and the associated anther size were also observed when the position of 35S:PAT was changed relative to 5126:DAM. These data provide evidence that gene expression in transformed maize plants can be impacted by simply altering the order, orientation or regulatory sequences of adjacent genes.
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