ArticleThe EMBO journal2001
Stepwise activation of the immunoglobulin mu heavy chain gene locus.
Article in The EMBO journal, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 70 papers.
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Who cites it
70 citing papers in PubMed.
- CRISPR-mediated precise large fragment insertion in zygotes enables rapid generation of humanized immunoglobulin heavy-chain mice.Immunity · 2026Article
- A hierarchy of spatial confinements governs chromatin dynamics and genomic encounters.bioRxiv : the preprint server for biology · 2026Article
- Non-canonical functions of enhancers: regulation of RNA polymerase III transcription, DNA replication, and V(D)J recombination.Trends in genetics : TIG · 2024Article
- Expression of the ether-a-gò-gò-related gene 1 channel during B and T lymphocyte development: role in BCR and TCR signaling.Frontiers in immunology · 2023Article
- IL-7R signaling activates widespread VCell reports · 2021Article
- Loss of H3K36 Methyltransferase SETD2 Impairs V(D)J Recombination during Lymphoid Development.iScience · 2020Article
- B-1a cells acquire their unique characteristics by bypassing the pre-BCR selection stage.Nature communications · 2019Article
- Chromosome dynamics near the sol-gel phase transition dictate the timing of remote genomic interactions.Nature communications · 2019Article
- New insights emerge as antibody repertoire diversification meets chromosome conformation.F1000Research · 2019Review
- Induced B Cell Development in Adult Mice.Frontiers in immunology · 2018Article
- Decreased IL7Rα and TdT expression underlie the skewed immunoglobulin repertoire of human B-cell precursors from fetal origin.Scientific reports · 2016Article
- Chromatin organization in pluripotent cells: emerging approaches to study and disrupt function.Briefings in functional genomics · 2016Review
- Dissecting Epigenetic Dysregulation of Primary Antibody Deficiencies.Journal of clinical immunology · 2016Review
- Histone methylation and V(D)J recombination.International journal of hematology · 2014Review
- Interleukin-7 and interleukin-15 for cancer.Journal of Cancer · 2014Review
- Opposing action of nuclear factor κB and Polo-like kinases determines a homeostatic end point for excitatory synaptic adaptation.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2013Article
- Deep sequencing of the murine IgH repertoire reveals complex regulation of nonrandom V gene rearrangement frequencies.Journal of immunology (Baltimore, Md. : 1950) · 2013Article
- Localized DNA demethylation at recombination intermediates during immunoglobulin heavy chain gene assembly.PLoS biology · 2013Article
- Localized epigenetic changes induced by DH recombination restricts recombinase to DJH junctions.Nature immunology · 2012Article
- IL-7 functionally segregates the pro-B cell stage by regulating transcription of recombination mediators across cell cycle.Journal of immunology (Baltimore, Md. : 1950) · 2012Article
10 more citing papers are in PubMed but not listed here.
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2 authors.
Funding
Abstract
The immunoglobulin heavy chain (IgH) gene locus spans several megabases. We show that IgH activation during B-cell differentiation, as measured by histone acetylation, occurs in discrete, independently regulated domains. Initially, a 120 kb domain of germline DNA is hyperacetylated, that extends from D(FL16.1), the 5'-most D(H) gene segment, to the intergenic region between Cmu and Cdelta. Germline V(H) genes were not hyperacetylated at this stage, which accounts for D(H) to J(H) recombination occurring first during B-cell development. Subsequent activation of the V(H) locus happens in at least three differentially regulated domains: an interleukin-7-regulated domain consisting of the 5' J558 family, an intermediate domain and the 3' V(H) genes, which are hyperacetylated in response to DJ(H) recombination. These observations lead to mechanisms for two well-documented phenomena in B-cell ontogeny: the sequential rearrangement of D(H) followed by V(H) gene segments, and the preferential recombination of D(H)-proximal V(H) genes in pro-B cells. We suggest that stepwise activation may be a general mechanism by which large segments of the genome are prepared for expression.
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