ArticleThe EMBO journal2001
The N-terminus of histone H2B, but not that of histone H3 or its phosphorylation, is essential for chromosome condensation.
Article in The EMBO journal, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
37 citing papers in PubMed, 90 citations in OpenAlex.
- Single amino-acid differences define H2B variants and modify chromatin accessibility to induce EMT in breast cancer.Oncogene · 2026Article
- Heat-Stress Impacts on Developing Bovine Oocytes: Unraveling Epigenetic Changes, Oxidative Stress, and Developmental Resilience.International journal of molecular sciences · 2024Article
- The Roles of Histone Post-Translational Modifications in the Formation and Function of a Mitotic Chromosome.International journal of molecular sciences · 2022Review
- Epigenetic landscape of testis specific histone H2B variant and its influence on sperm function.Clinical epigenetics · 2021Article
- Epigenetic Molecular Mechanisms in Insects.Neotropical entomology · 2020Review
- Priming chromatin for segregation: functional roles of mitotic histone modifications.Cell cycle (Georgetown, Tex.) · 2020Review
- Generation of Remosomes by the SWI/SNF Chromatin Remodeler Family.Scientific reports · 2019Article
- MST2 kinase suppresses rDNA transcription in response to DNA damage by phosphorylating nucleolar histone H2B.The EMBO journal · 2018Article
- Phosphorylation of histone H3 on Ser-10 by Aurora B is essential for chromosome condensation in porcine embryos during the first mitotic division.Histochemistry and cell biology · 2017Article
- Epigenetic countermarks in mitotic chromosome condensation.Nucleus (Austin, Tex.) · 2017Review
- FACT Assists Base Excision Repair by Boosting the Remodeling Activity of RSC.PLoS genetics · 2016Article
- Article
- Citrullination of DNMT3A by PADI4 regulates its stability and controls DNA methylation.Nucleic acids research · 2014Article
- Mitotic phosphorylation of histone H3 threonine 80.Cell cycle (Georgetown, Tex.) · 2014Article
- Basal aurora kinase B activity is sufficient for histone H3 phosphorylation in prophase.Biology open · 2013Article
- Contribution of histone N-terminal tails to the structure and stability of nucleosomes.FEBS open bio · 2013Article
- Lasonolide A, a potent and reversible inducer of chromosome condensation.Cell cycle (Georgetown, Tex.) · 2012Article
- Histone phosphorylation: a chromatin modification involved in diverse nuclear events.Epigenetics · 2012Review
- Mutagenesis of pairwise combinations of histone amino-terminal tails reveals functional redundancy in budding yeast.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Base excision repair of 8-oxoG in dinucleosomes.Nucleic acids research · 2012Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have studied the role of individual histone N-termini and the phosphorylation of histone H3 in chromosome condensation. Nucleosomes, reconstituted with histone octamers containing different combinations of recombinant full-length and tailless histones, were used as competitors for chromosome assembly in Xenopus egg extracts. Nucleosomes reconstituted with intact octamers inhibited chromosome condensation as efficiently as the native ones, while tailless nucleosomes were unable to affect this process. Importantly, the addition to the extract of particles containing only intact histone H2B strongly interfered with chromosome formation while such an effect was not observed with particles lacking the N-terminal tail of H2B. This demonstrates that the inhibition effect observed in the presence of competitor nucleosomes is mainly due to the N-terminus of this histone, which, therefore, is essential for chromosome condensation. Nucleosomes in which all histones but H3 were tailless did not impede chromosome formation. In addition, when competitor nucleosome particles were reconstituted with full-length H2A, H2B and H4 and histone H3 mutated at the phosphorylable serine 10 or serine 28, their inhibiting efficiency was identical to that of the native particles. Hence, the tail of H3, whether intact or phosphorylated, is not important for chromosome condensation. A novel hypothesis, termed 'the ready production label' was suggested to explain the role of histone H3 phosphorylation during cell division.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.