Evidence map›Paper›PMID 11707406›Full record

ArticleThe EMBO journal2001

Proteomic and functional evidence for a P2X7 receptor signalling complex.

M Kim, L H Jiang, H L Wilson, R A North, A Surprenant

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The EMBO journal, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 156 papers.

0numbers the graph read from it
0cells of the map it votes in
156citing papers in PubMed
11.4field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

156 citing papers in PubMed, 377 citations in OpenAlex.

  1. Article
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  14. P2YMolecules (Basel, Switzerland) · 2021
    Review
  15. Review
  16. Review
  17. Review
  18. P2XFrontiers in cellular neuroscience · 2021
    Review
  19. Review
  20. Article

96 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

M KimInstitute of Molecular Physiology, University of Sheffield, Alfred Denny Building, Western Bank, Sheffield S10 2TN, UK.
L H Jiang
H L Wilson
R A North
A Surprenant
University of Sheffield · GB

Funding

Wellcome Trust
6 · The paper itself

Abstract

P2X receptors are ATP-gated ion channels in the plasma membrane, but activation of the P2X7 receptor also leads to rapid cytoskeletal re-arrangements such as membrane blebbing. We identified 11 proteins in human embryonic kidney cells that interact with the rat P2X7 receptor, by affinity purification followed by mass spectroscopy and immunoblotting [laminin alpha3, integrin beta2, beta-actin, alpha-actinin, supervillin, MAGuK, three heat shock proteins, phosphatidylinositol 4-kinase and receptor protein tyrosine phosphatase-beta (RPTPbeta)]. Activation of the P2X7 receptor resulted in its dephosphorylation. Whole-cell recordings from cells expressing P2X7 receptors showed that this markedly reduced subsequent ionic currents and it also slowed membrane bleb formation. By mutagenesis, we identified Tyr(343) in the putative second transmembrane domain as the site of phosphorylation. Thus, we have identified a P2X7 receptor signalling complex, some members of which may initiate cytoskeletal rearrangements following receptor activation. Others, such as RPTPbeta, might exert feedback control of the channel itself through its dephosphorylation.

Indexed as

Adenosine TriphosphateAnimalsBlotting, WesternCell MembraneCytoskeletonDNA, ComplementaryHumansImmunoblottingKidneyModels, BiologicalMutagenesis, Site-DirectedMutationPhosphorylationPrecipitin TestsRatsReceptors, Purinergic P2Adenosine TriphosphateDNA, ComplementaryP2RX7 protein, humanP2rx7 protein, ratReceptors, Purinergic P2Receptors, Purinergic P2X7Tyrosine

Identifiers

PMID11707406
PMCPMC125721
OpenAlexW2099719313

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.