ArticleThe EMBO journal2001
Asymmetrical, agonist-induced fluctuations in local extracellular [Ca(2+)] in intact polarized epithelia.
Article in The EMBO journal, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
18 citing papers in PubMed, 59 citations in OpenAlex.
- Fluorescent sensors for imaging of interstitial calcium.Nature communications · 2023Article
- Calcium-sensing receptor is not expressed in the absorptive enterocytes of weaned piglets.Journal of animal science · 2022Article
- Calcium-sensing receptor-mediated NLRP3 inflammasome response to calciprotein particles drives inflammation in rheumatoid arthritis.Nature communications · 2020Article
- The Different Facets of Extracellular Calcium Sensors: Old and New Concepts in Calcium-Sensing Receptor Signalling and Pharmacology.International journal of molecular sciences · 2018Review
- Luminal ANG II is internalized as a complex with ATAmerican journal of physiology. Renal physiology · 2017Article
- Calcium-sensing receptor: A new target for therapy of diarrhea.World journal of gastroenterology · 2016Review
- Recent advances in understanding the extracellular calcium-sensing receptor.F1000Research · 2016Review
- The Extracellular Calcium-Sensing Receptor in the Intestine: Evidence for Regulation of Colonic Absorption, Secretion, Motility, and Immunity.Frontiers in physiology · 2016Review
- "Store-operated" cAMP signaling contributes to Ca2+-activated Cl- secretion in T84 colonic cells.American journal of physiology. Gastrointestinal and liver physiology · 2015Article
- Importance of Ca(2+) in gastric epithelial restitution-new views revealed by real-time in vivo measurements.Current opinion in pharmacology · 2014Review
- In vivo epithelial wound repair requires mobilization of endogenous intracellular and extracellular calcium.The Journal of biological chemistry · 2013Article
- The parietal cell gastric H, K-ATPase also functions as the Na, K-ATPase and Ca-ATPase in altered states.F1000Research · 2013Article
- Neurochemical characterization of zinc transporter 3-like immunoreactive (ZnT3(+)) neurons in the intramural ganglia of the porcine duodenum.Journal of molecular neuroscience : MN · 2012Article
- Demand for Zn2+ in acid-secreting gastric mucosa and its requirement for intracellular Ca2+.PloS one · 2011Article
- Regulation of calcium-sensing-receptor trafficking and cell-surface expression by GPCRs and RAMPs.Trends in pharmacological sciences · 2008Review
- Extracellular calcium as an integrator of tissue function.The international journal of biochemistry & cell biology · 2008Review
- Role of calcium and other trace elements in the gastrointestinal physiology.World journal of gastroenterology · 2006Review
- Extracellular calcium acts as a "third messenger" to regulate enzyme and alkaline secretion.The Journal of cell biology · 2004Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 1 institution in 1 country.
Funding
Abstract
We recently proposed that extracellular Ca(2+) ions participate in a novel form of intercellular communication involving the extracellular Ca(2+)-sensing receptor (CaR). Here, using Ca(2+)-selective microelectrodes, we directly measured the profile of agonist-induced [Ca(2+)]ext changes in restricted domains near the basolateral or luminal membranes of polarized gastric acid-secreting cells. The Ca(2+)-mobilizing agonist carbachol elicited a transient, La(3+)-sensitive decrease in basolateral [Ca(2+)] (average approximately 250 microM, but as large as 530 microM). Conversely, carbachol evoked an HgCl2-sensitive increase in [Ca(2+)] (average approximately 400 microM, but as large as 520 microM) in the lumen of single gastric glands. Both responses were significantly reduced by pre-treatment with sarco-endoplasmic reticulum Ca(2+) ATPase (SERCA) pump inhibitors or with the intracellular Ca(2+) chelator BAPTA-AM. Immunofluorescence experiments demonstrated an asymmetric localization of plasma membrane Ca(2+) ATPase (PMCA), which appeared to be partially co-localized with CaR and the gastric H(+)/K(+)-ATPase in the apical membrane of the acid-secreting cells. Our data indicate that agonist stimulation results in local fluctuations in [Ca(2+)]ext that would be sufficient to modulate the activity of the CaR on neighboring cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.