ArticleThe EMBO journal2001
Structure of a fast kinesin: implications for ATPase mechanism and interactions with microtubules.
Article in The EMBO journal, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
27 citing papers in PubMed.
- NovelBalkan journal of medical genetics : BJMG · 2025Article
- Unraveling the interplay of kinesin-1, tau, and microtubules in neurodegeneration associated with Alzheimer's disease.Frontiers in cellular neuroscience · 2024Review
- Kinesin Motors in the Filamentous Basidiomycetes in Light of theJournal of fungi (Basel, Switzerland) · 2022Review
- Absence of DEATH kinesin is fatal for Leishmania mexicana amastigotes.Scientific reports · 2022Article
- Shaft Function of Kinesin-1's α4 Helix in the Processive Movement.Cellular and molecular bioengineering · 2019Article
- KIF5A transports collagen vesicles of myofibroblasts during pleural fibrosis.Scientific reports · 2017Article
- Kinesin-2 KIF3AC and KIF3AB Can Drive Long-Range Transport along Microtubules.Biophysical journal · 2015Article
- Kinesin Motor Enzymology: Chemistry, Structure, and Physics of Nanoscale Molecular Machines.Biophysical reviews · 2015Article
- Kinesin, 30 years later: Recent insights from structural studies.Protein science : a publication of the Protein Society · 2015Review
- The crystal structure and biochemical characterization of Kif15: a bifunctional molecular motor involved in bipolar spindle formation and neuronal development.Acta crystallographica. Section D, Biological crystallography · 2014Article
- Preferential binding of a kinesin-1 motor to GTP-tubulin-rich microtubules underlies polarized vesicle transport.The Journal of cell biology · 2011Article
- Multiple conformations of the nucleotide site of Kinesin family motors in the triphosphate state.Journal of molecular biology · 2011Article
- Structures of kinesin motor proteins.Cell motility and the cytoskeleton · 2009Review
- Insights into the Mechanical Properties of the Kinesin Neck Linker Domain from Sequence Analysis and Molecular Dynamics Simulations.Cellular and molecular bioengineering · 2009Article
- The Aspergillus nidulans kinesin-3 UncA motor moves vesicles along a subpopulation of microtubules.Molecular biology of the cell · 2009Article
- Vik1 modulates microtubule-Kar3 interactions through a motor domain that lacks an active site.Cell · 2007Article
- Interaction of kinesin motors, microtubules, and MAPs.Journal of muscle research and cell motility · 2006Review
- Back on track - on the role of the microtubule for kinesin motility and cellular function.Journal of muscle research and cell motility · 2006Review
- Review: regulation mechanisms of Kinesin-1.Journal of muscle research and cell motility · 2006Review
- Probing the local dynamics of nucleotide-binding pocket coupled to the global dynamics: myosin versus kinesin.Biophysical journal · 2005Article
Corrections and comments
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We determined the crystal structure of the motor domain of the fast fungal kinesin from Neurospora crassa (NcKin). The structure has several unique features. (i) Loop 11 in the switch 2 region is ordered and enables one to describe the complete nucleotide-binding pocket, including three inter-switch salt bridges between switch 1 and 2. (ii) Loop 9 in the switch 1 region bends outwards, making the nucleotide-binding pocket very wide. The displacement in switch 1 resembles that of the G-protein ras complexed with its guanosine nucleotide exchange factor. (iii) Loop 5 in the entrance to the nucleotide-binding pocket is remarkably long and interacts with the ribose of ATP. (iv) The linker and neck region is not well defined, indicating that it is mobile. (v) Image reconstructions of ice-embedded microtubules decorated with NcKin show that it interacts with several tubulin subunits, including a central beta-tubulin monomer and the two flanking alpha-tubulin monomers within the microtubule protofilament. Comparison of NcKin with other kinesins, myosin and G-proteins suggests that the rate-limiting step of ADP release is accelerated in the fungal kinesin and accounts for the unusually high velocity and ATPase activity.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.