ArticlePlant physiology2001
Rapid deposition of extensin during the elicitation of grapevine callus cultures is specifically catalyzed by a 40-kilodalton peroxidase.
Article in Plant physiology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
29 citing papers in PubMed, 88 citations in OpenAlex.
- Endophytic mycorrhizal fungi strengthen Lactuca sativa defense against Alternaria alternata as a sustainable biocontrol approach.BMC plant biology · 2026Article
- GLRaV-2 protein p24 suppresses host defenses by interaction with a RAV transcription factor from grapevine.Plant physiology · 2022Article
- Class III Peroxidases PRX01, PRX44, and PRX73 Control Root Hair Growth inInternational journal of molecular sciences · 2022Article
- Apoplastic class III peroxidases PRX62 and PRX69 promote Arabidopsis root hair growth at low temperature.Nature communications · 2022Article
- Extensins: Self-Assembly, Crosslinking, and the Role of Peroxidases.Frontiers in plant science · 2021Review
- Exploring the Use of Fruit Callus Culture as a Model System to Study Color Development and Cell Wall Remodeling during Strawberry Fruit Ripening.Plants (Basel, Switzerland) · 2020Article
- Metabolomic Fingerprinting of Potato Cultivars Differing in Susceptibility to Spongospora subterranea f. sp. subterranea Root Infection.International journal of molecular sciences · 2020Article
- Extensin arabinosylation is involved in root response to elicitors and limits oomycete colonization.Annals of botany · 2020Article
- Review
- Candidate Gene Networks for Acylsugar Metabolism and Plant Defense in Wild TomatoThe Plant cell · 2020Article
- PRX9 and PRX40 Are Extensin Peroxidases Essential for Maintaining Tapetum and Microspore Cell Wall Integrity during Arabidopsis Anther Development.The Plant cell · 2019Article
- Immunolocalization and Changes of Hydroxyproline-Rich Glycoproteins During Symbiotic Germination ofFrontiers in plant science · 2018Article
- How the Depletion in Mineral Major Elements Affects Grapevine (Frontiers in plant science · 2017Review
- The effects of exogenous hormones on rooting process and the activities of key enzymes of Malus hupehensis stem cuttings.PloS one · 2017Article
- Low Sugar Is Not Always Good: Impact of Specific O-Glycan Defects on Tip Growth in Arabidopsis.Plant physiology · 2015Article
- Identification of the pI 4.6 extensin peroxidase from Lycopersicon esculentum using proteomics and reverse-genomics.Phytochemistry · 2015Article
- Characterization of the serine acetyltransferase gene family of Vitis vinifera uncovers differences in regulation of OAS synthesis in woody plants.Frontiers in plant science · 2015Article
- Role of proline in cell wall synthesis and plant development and its implications in plant ontogeny.Frontiers in plant science · 2015Review
- Pectic-β(1,4)-galactan, extensin and arabinogalactan-protein epitopes differentiate ripening stages in wine and table grape cell walls.Annals of botany · 2014Article
- Cell wall O-glycoproteins and N-glycoproteins: aspects of biosynthesis and function.Frontiers in plant science · 2014Review
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Elicitation or peroxide stimulation of grape (Vitis vinifera L. cv Touriga) vine callus cultures results in the rapid and selective in situ insolubilization of an abundant and ionically bound cell wall protein-denominated GvP1. Surface-enhanced laser desorption/ionization/time of flight-mass spectrometry analysis, the amino acid composition, and the N-terminal sequence of purified GvP1 identified it as an 89.9-kD extensin. Analysis of cell walls following the in situ insolubilization of GvP1 indicates large and specific increases in the major amino acids of GvP1 as compared with the amino acids present in salt-eluted cell walls. We calculate that following deposition, covalently bound GvP1 contributes up to 4% to 5% of the cell wall dry weight. The deposition of GvP1 in situ requires peroxide and endogenous peroxidase activity. Isoelectric focusing of saline eluates of callus revealed only a few basic peroxidases that were all isolated or purified to electrophoretic homogeneity. In vitro and in situ assays of extensin cross-linking activity using GvP1 and peroxidases showed that a 40-kD peroxidase cross-linked GvP1 within minutes, whereas other grapevine peroxidases had no significant activity with GvP1. Internal peptide sequences indicated this extensin peroxidase (EP) is a member of the class III peroxidases. We conclude that we have identified and purified an EP from grapevine callus that is responsible for the catalysis of GvP1 deposition in situ during elicitation. Our results suggest that GvP1 and this EP play an important combined role in grapevine cell wall defense.
Indexed as
Identifiers
11706187PMC129276W1992058985What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.