ArticlePlant physiology2001
Chloroplast transcription at different light intensities. Glutathione-mediated phosphorylation of the major RNA polymerase involved in redox-regulated organellar gene expression.
Article in Plant physiology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 66 citations in OpenAlex.
- Circadian and environmental signal integration in a natural population ofProceedings of the National Academy of Sciences of the United States of America · 2024Article
- Photosynthesis in a Changing Global Climate: Scaling Up and Scaling Down in Crops.Frontiers in plant science · 2020Review
- Contrasting growth, physiological and gene expression responses of Clematis crassifolia and Clematis cadmia to different irradiance conditions.Scientific reports · 2019Article
- Plant Glutathione Transferases and Light.Frontiers in plant science · 2018Review
- Post-translational Modifications in Regulation of Chloroplast Function: Recent Advances.Frontiers in plant science · 2017Review
- Comparative Analysis of ChloroplastFrontiers in plant science · 2017Article
- Organellar Gene Expression and Acclimation of Plants to Environmental Stress.Frontiers in plant science · 2017Review
- Integration of light and circadian signals that regulate chloroplast transcription by a nuclear-encoded sigma factor.The New phytologist · 2017Article
- Evolutionary rewiring: a modified prokaryotic gene-regulatory pathway in chloroplasts.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2013Article
- Signal integration by chloroplast phosphorylation networks: an update.Frontiers in plant science · 2012Article
- Acclimation to high-light conditions in cyanobacteria: from gene expression to physiological responses.Journal of plant research · 2012Review
- A glutathione S-transferase regulated by light and hormones participates in the modulation of Arabidopsis seedling development.Plant physiology · 2010Article
- AtSIG6, a plastid sigma factor from Arabidopsis, reveals functional impact of cpCK2 phosphorylation.The Plant journal : for cell and molecular biology · 2010Article
- Transcriptional control of photosynthesis genes: the evolutionarily conserved regulatory mechanism in plastid genome function.Genome biology and evolution · 2010Article
- Conformational changes in redox pairs of protein structures.Protein science : a publication of the Protein Society · 2009Article
- Plant sigma factors and their role in plastid transcription.Plant cell reports · 2007Review
- Nucleus-encoded plastid sigma factor SIG3 transcribes specifically the psbN gene in plastids.Nucleic acids research · 2007Article
- Glutathione homeostasis and redox-regulation by sulfhydryl groups.Photosynthesis research · 2005Review
- Glutathione, photosynthesis and the redox regulation of stress-responsive gene expression.Photosynthesis research · 2005Review
- The activity of the chloroplastic Ndh complex is regulated by phosphorylation of the NDH-F subunit.Plant physiology · 2003Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Previous studies using purified RNA polymerase from mustard (Sinapis alba) chloroplasts showed control of transcription by an associated protein kinase. This kinase was found to respond to reversible thiol/disulfide formation mediated by glutathione (GSH), although at concentrations exceeding those thought to exist in vivo. In the present study, several lines of evidence are presented to substantiate the functioning of this regulation mechanism, also in vivo: (a) Studies on the polymerase-associated transcription kinase revealed that at appropriate ATP levels, GSH concentrations similar to those in vivo are sufficient to modulate the kinase activity; (b) GSH measurements from isolated mustard chloroplasts showed considerable differences in response to light intensity; (c) this was reflected by run-on transcription rates in isolated chloroplasts that were generally higher if organelles were prepared from seedlings incubated under high-light as compared with growth-light conditions; (d) the notion of a general transcriptional switch was strengthened by in vitro experiments showing that the kinase not only affects the transcription of a photosynthetic gene (psbA) but also that of a non-photosynthetic gene (trnQ); and (e) the polymerase-kinase complex revealed specific differences in the phosphorylation state of polypeptides depending on the light intensity to which the seedlings had been exposed prior to chloroplast isolation. Taken together, these data are consistent with GSH and phosphorylation-dependent regulation of chloroplast transcription in vivo.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.