ArticleThe Journal of cell biology2001
Myosin V exhibits a high duty cycle and large unitary displacement.
Article in The Journal of cell biology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
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Who cites it
43 citing papers in PubMed, 85 citations in OpenAlex.
- Research reviews on myosin head interactions with F-actin.Applied microscopy · 2024Review
- Philament: A filament tracking program to quickly and accurately analyze in vitro motility assays.Biophysical reports · 2024Article
- Acetylation of fission yeast tropomyosin does not promote differential association with cognate formins.Cytoskeleton (Hoboken, N.J.) · 2023Article
- Functional Role of Class III Myosins in Hair Cells.Frontiers in cell and developmental biology · 2021Review
- Measuring Stepwise Binding of Thermally Fluctuating Particles to Cell Membranes without Fluorescence.Biophysical journal · 2020Article
- HCM and DCM cardiomyopathy-linked α-tropomyosin mutations influence off-state stability and crossbridge interaction on thin filaments.Archives of biochemistry and biophysics · 2018Article
- Omecamtiv Mecarbil Enhances the Duty Ratio of Human β-Cardiac Myosin Resulting in Increased Calcium Sensitivity and Slowed Force Development in Cardiac Muscle.The Journal of biological chemistry · 2017Article
- Strain Mediated Adaptation Is Key for Myosin Mechanochemistry: Discovering General Rules for Motor Activity.PLoS computational biology · 2016Article
- Biological machines: Molecular motor teamwork.Nature nanotechnology · 2015Article
- Cell-sized spherical confinement induces the spontaneous formation of contractile actomyosin rings in vitro.Nature cell biology · 2015Article
- Impact of familial hypertrophic cardiomyopathy-linked mutations in the NH2 terminus of the RLC on β-myosin cross-bridge mechanics.Journal of applied physiology (Bethesda, Md. : 1985) · 2014Article
- Myosin-10 produces its power-stroke in two phases and moves processively along a single actin filament under low load.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Superresolution imaging of dynamic MreB filaments in B. subtilis--a multiple-motor-driven transport?Biophysical journal · 2013Article
- Breaking the millisecond barrier: single molecule motors wobble to find their next binding sites.Biophysical journal · 2013Article
- Global fit analysis of myosin-5b motility reveals thermodynamics of Mg2+-sensitive acto-myosin-ADP states.PloS one · 2013Article
- Temperature dependent measurements reveal similarities between muscle and non-muscle myosin motility.Journal of muscle research and cell motility · 2012Article
- Calcium-induced mechanical change in the neck domain alters the activity of plant myosin XI.The Journal of biological chemistry · 2012Article
- Understanding cardiomyopathy phenotypes based on the functional impact of mutations in the myosin motor.Circulation research · 2012Review
- Switching of myosin-V motion between the lever-arm swing and brownian search-and-catch.Nature communications · 2012Article
- Coarse-grained simulation of myosin-V movement.Computational and mathematical methods in medicine · 2012Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Myosin V is a double-headed unconventional myosin that has been implicated in organelle transport. To perform this role, myosin V may have a high duty cycle. To test this hypothesis and understand the properties of this molecule at the molecular level, we used the laser trap and in vitro motility assay to characterize the mechanics of heavy meromyosin-like fragments of myosin V (M5(HMM)) expressed in the Baculovirus system. The relationship between actin filament velocity and the number of interacting M5(HMM) molecules indicates a duty cycle of > or =50%. This high duty cycle would allow actin filament translocation and thus organelle transport by a few M5(HMM) molecules. Single molecule displacement data showed predominantly single step events of 20 nm and an occasional second step to 37 nm. The 20-nm unitary step represents the myosin V working stroke and is independent of the mode of M5(HMM) attachment to the motility surface or light chain content. The large M5(HMM) working stroke is consistent with the myosin V neck acting as a mechanical lever. The second step is characterized by an increased displacement variance, suggesting a model for how the two heads of myosin V function in processive motion.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.