ArticleThe Journal of cell biology2001
Filamin A, the Arp2/3 complex, and the morphology and function of cortical actin filaments in human melanoma cells.
Article in The Journal of cell biology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 87 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
87 citing papers in PubMed, 193 citations in OpenAlex.
- Ion transport as a determinant of membrane fusion and fission.Nature structural & molecular biology · 2026Review
- Crosslinked F-actin networks regulate load-dependent energy conversion.Communications biology · 2026Article
- Unraveling the pathogenic mechanism of a novel filamin a frameshift variant in periventricular nodular heterotopia.Frontiers in pharmacology · 2024Article
- Intracellular tension sensor reveals mechanical anisotropy of the actin cytoskeleton.Nature communications · 2023Article
- Calcium bursts allow rapid reorganization of EFhD2/Swip-1 cross-linked actin networks in epithelial wound closure.Nature communications · 2022Article
- Review
- Flying under the radar: CDH2 (N-cadherin), an important hub molecule in neurodevelopmental and neurodegenerative diseases.Frontiers in neuroscience · 2022Review
- Plastin and spectrin cooperate to stabilize the actomyosin cortex during cytokinesis.Current biology : CB · 2021Article
- Filamin A Is Required for NK Cell Cytotoxicity at the Expense of Cytokine ProductionFrontiers in immunology · 2021Article
- Role and mechanism of FLNa and UCP2 in the development of cervical cancer.Oncology reports · 2020Observational
- Predictive assembling model reveals the self-adaptive elastic properties of lamellipodial actin networks for cell migration.Communications biology · 2020Article
- FLN-1/filamin is required to anchor the actomyosin cytoskeleton and for global organization of sub-cellular organelles in a contractile tissue.Cytoskeleton (Hoboken, N.J.) · 2020Article
- p21-Activated Kinases in Thyroid Cancer.Endocrinology · 2020Review
- Proteomic Profiling of Retinoblastoma-Derived Exosomes Reveals Potential Biomarkers of Vitreous Seeding.Cancers · 2020Article
- Clinicopathological significance of immunohistochemical expression of Filamin A in breast cancer.Journal of carcinogenesis · 2020Article
- Inducible microRNA-200c decreases motility of breast cancer cells and reduces filamin A.PloS one · 2019Article
- Filamin A inhibits tumor progression through regulating BRCA1 expression in human breast cancer.Oncology letters · 2018Article
- Varicose and cheerio collaborate with pebble to mediate semaphorin-1a reverse signaling inProceedings of the National Academy of Sciences of the United States of America · 2017Article
- Identification of oral cancer related candidate genes by integrating protein-protein interactions, gene ontology, pathway analysis and immunohistochemistry.Scientific reports · 2017Article
- Apical Sarcomere-like Actomyosin Contracts Nonmuscle Drosophila Epithelial Cells.Developmental cell · 2016Article
27 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
The Arp2/3 complex and filamin A (FLNa) branch actin filaments. To define the role of these actin-binding proteins in cellular actin architecture, we compared the morphology of FLNa-deficient human melanoma (M2) cells and three stable derivatives of these cells expressing normal FLNa concentrations. All the cell lines contain similar amounts of the Arp2/3 complex. Serum addition causes serum-starved M2 cells to extend flat protrusions transiently; thereafter, the protrusions turn into spherical blebs and the cells do not crawl. The short-lived lamellae of M2 cells contain a dense mat of long actin filaments in contrast to a more three-dimensional orthogonal network of shorter actin filaments in lamellae of identically treated FLNa-expressing cells capable of translational locomotion. FLNa-specific antibodies localize throughout the leading lamellae of these cells at junctions between orthogonally intersecting actin filaments. Arp2/3 complex-specific antibodies stain diffusely and label a few, although not the same, actin filament overlap sites as FLNa antibody. We conclude that FLNa is essential in cells that express it for stabilizing orthogonal actin networks suitable for locomotion. Contrary to some proposals, Arp2/3 complex-mediated branching of actin alone is insufficient for establishing an orthogonal actin organization or maintaining mechanical stability at the leading edge.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.