ArticleInfection and immunity2001
Mycobacterial protein HbhA binds human complement component C3.
Article in Infection and immunity, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
24 citing papers in PubMed, 49 citations in OpenAlex.
- Complement C3 and Activated Fragment C3a Are Involved in Complement Activation and Anti-Bacterial Immunity.Frontiers in immunology · 2022Article
- Article
- Innate Immune Pattern Recognition Receptors of Mycobacterium tuberculosis: Nature and Consequences for Pathogenesis of Tuberculosis.Advances in experimental medicine and biology · 2021Article
- TheThe Biochemical journal · 2019Review
- A Two-Way Proteome Microarray Strategy to Identify NovelFrontiers in cellular and infection microbiology · 2019Article
- Association of Mycobacterium Proteins with Lipid Droplets.Journal of bacteriology · 2018Article
- Complement Dependent and Independent Interaction Between Bovine Conglutinin andFrontiers in immunology · 2018Article
- Longitudinal Evaluation of Humoral Immunity and Bacterial and Clinical Parameters Reveals That Antigen-Specific Antibodies Suppress Inflammatory Responses in Active Tuberculosis Patients.Journal of immunology research · 2018Article
- Systematic Identification ofMolecular & cellular proteomics : MCP · 2017Article
- Article
- Identification of OprF as a complement component C3 binding acceptor molecule on the surface of Pseudomonas aeruginosa.Infection and immunity · 2015Article
- Binding of CXCL8/IL-8 to Mycobacterium tuberculosis Modulates the Innate Immune Response.Mediators of inflammation · 2015Article
- Article
- Targeting of Mycobacterium tuberculosis heparin-binding hemagglutinin to mitochondria in macrophages.PLoS pathogens · 2011Article
- Bacillus anthracis interacts with plasmin(ogen) to evade C3b-dependent innate immunity.PloS one · 2011Article
- Biochemical and physiological characterization of the GTP-binding protein Obg of Mycobacterium tuberculosis.BMC microbiology · 2011Article
- Mycobacterium avium uses apoptotic macrophages as tools for spreading.Microbial pathogenesis · 2011Article
- Methionine sulfoxide reductase B (MsrB) of Mycobacterium smegmatis plays a limited role in resisting oxidative stress.Tuberculosis (Edinburgh, Scotland) · 2009Article
- Dichotomous role of the macrophage in early Mycobacterium marinum infection of the zebrafish.Cell host & microbe · 2007Article
- Mycobacterium tuberculosis HBHA protein reacts strongly with the serum immunoglobulin M of tuberculosis patients.Clinical and vaccine immunology : CVI · 2006Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Mycobacterium tuberculosis and Mycobacterium avium are facultative intracellular pathogens that are able to survive and replicate in mononuclear phagocytes. Human complement component C3 has previously been shown to mediate attachment and phagocytosis of these bacteria by mononuclear phagocytes. In this study, a C3 ligand affinity blot protocol was used to identify a 30-kDa C3-binding protein in M. tuberculosis and Mycobacterium smegmatis and a 31-kDa C3-binding protein in M. avium. The C3-binding proteins in M. tuberculosis and M. avium localized to the cell membrane fraction and partitioned to the detergent fraction during Triton X-114 phase partitioning. The C3-binding protein from M. tuberculosis was partially purified using a cation exchange column and was shown to bind concanavalin A. The N terminus and an internal fragment of the partially purified C3-binding protein were subjected to amino acid sequence analysis. The resulting amino acid sequences matched the M. tuberculosis heparin-binding hemagglutinin (HbhA) protein. Recombinant full-length HbhA and the C terminus of HbhA fused to maltose-binding protein, but not recombinant HbhA lacking the C-terminal region, bound human C3. Recombinant full-length HbhA coated on polystyrene beads, was found to enhance the adherence and/or phagocytosis of the coated beads to J774.A1 cells in both the presence and absence of human serum. The presence of complement-sufficient serum increased the adherence of the HbhA-coated beads to the J774.A1 cells in a C3-dependent manner. If HbhA within the bacterial cell membrane functions similarly to isolated HbhA, this protein may enhance the adherence and phagocytosis of M. tuberculosis and M. avium to mononuclear phagocytes through the binding of C3 and interaction with C3 receptors on mononuclear phagocytes.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.