ArticleInfection and immunity2001
Intranasal immunization with recombinant Ascaris suum 14-kilodalton antigen coupled with cholera toxin B subunit induces protective immunity to A. suum infection in mice.
Article in Infection and immunity, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers, 1 of them a synthesis that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
32 citing papers in PubMed, 1 synthesis or guideline pooled it, 76 citations in OpenAlex.
- A historical and systematic overview ofParasitology · 2021Pooled it
- Hookworm genes encoding intestinal excreted-secreted proteins are transcriptionally upregulated in response to the host's immune system.PLoS neglected tropical diseases · 2026Article
- In silico design of a polypeptide as a vaccine candidate against ascariasis.Scientific reports · 2023Article
- Identification of broadly-conserved parasitic nematode proteins that activate immunity.Frontiers in parasitology · 2023Article
- Advancing a Human Onchocerciasis Vaccine From Antigen Discovery to Efficacy Studies Against Natural Infection of Cattle WithFrontiers in cellular and infection microbiology · 2022Review
- A reverse vaccinology approach identifies putative vaccination targets in the zoonotic nematodeFrontiers in veterinary science · 2022Article
- ASCVac-1, a Multi-Peptide Chimeric Vaccine, Protects Mice AgainstFrontiers in immunology · 2021Article
- Rodent Models for the Study of Soil-Transmitted Helminths: A Proteomics Approach.Frontiers in cellular and infection microbiology · 2021Review
- Identification and Physicochemical Characterization of a New Allergen fromInternational journal of molecular sciences · 2020Article
- Whipworm and roundworm infections.Nature reviews. Disease primers · 2020Review
- Protective immunity elicited by the nematode-conserved As37 recombinant protein against Ascaris suum infection.PLoS neglected tropical diseases · 2020Article
- Anthelmintic drug discovery: target identification, screening methods and the role of open science.Beilstein journal of organic chemistry · 2020Review
- Soil-Transmitted Helminth Vaccines: Are We Getting Closer?Frontiers in immunology · 2020Review
- Vaccination Against Whipworm: Identification of Potential Immunogenic Proteins in Trichuris muris Excretory/Secretory Material.Scientific reports · 2018Article
- Parasites of the Giant Panda: A Risk Factor in the Conservation of a Species.Advances in parasitology · 2018Review
- Onchocerca volvulus: The Road from Basic Biology to a Vaccine.Trends in parasitology · 2018Review
- IgG Induced by Vaccination WithFrontiers in immunology · 2018Article
- Recombinant subunit vaccines for soil-transmitted helminths.Parasitology · 2017Review
- Yeast-expressed recombinant As16 protects mice against Ascaris suum infection through induction of a Th2-skewed immune response.PLoS neglected tropical diseases · 2017Article
- The Immunomodulatory Role of Adjuvants in Vaccines Formulated with the Recombinant Antigens Ov-103 and Ov-RAL-2 against Onchocerca volvulus in Mice.PLoS neglected tropical diseases · 2016Article
Corrections and comments
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Authors and funding
7 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Animals can be rendered immune to Ascaris parasites by immunization with infectious-stage larvae. The specific parasite gene products that mediate protective responses in ascariasis are unknown. We have identified a cDNA encoding Ascaris suum 14-kDa antigen (As14) and evaluated the vaccinal effect of the Escherichia coli-expressed recombinant protein (rAs14). GenBank analysis showed that As14 has low similarity at the amino acid level to a Caenorhabditis elegans gene product and to antigens of the filarial nematodes but not to other known proteins. In addition, As14 homologues were found to be expressed in human and dog roundworms. In mice that received intranasal administration of rAs14 coupled with cholera toxin B subunit (rAs14-CTB), there was a 64% reduction of recovery of larvae compared with that in the nontreated group. The vaccinated mice showed a significant increase in the total serum immunoglobulin G (IgG) levels and the mucosal IgA responses. Elevation of the rAs14-specific IgE response was also seen. Measurement of the IgG subclasses showed a higher level of IgG1 and a lower level of IgG2a antibody response in the sera of the immunized mice, suggesting that protection was associated with a type II immune response. As14 is the first protective antigen against A. suum infection to be identified. Our immunization trial results in laboratory animals suggest the possibility of developing a mucosal vaccine for parasitic diseases caused by ascarid nematodes.
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