ArticleProceedings of the National Academy of Sciences of the United States of America2001
UniGene cDNA array-based monitoring of transcriptome changes during mouse placental development.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 48 citations in OpenAlex.
- Single-cell RNA sequencing identifies CXADR as a fate determinant of the placental exchange surface.Nature communications · 2025Article
- A single cell transcriptional portrait of embryoid body differentiation and comparison to progenitors of the developing embryo.Stem cell research · 2018Article
- Expression and function of nuclear receptor co-activator 4: evidence of a potential role independent of co-activator activity.Cellular and molecular life sciences : CMLS · 2012Review
- Identification of maternally regulated fetal gene networks in the placenta with a novel embryo transfer system in mice.Physiological genomics · 2011Article
- The role of placental homeobox genes in human fetal growth restriction.Journal of pregnancy · 2011Review
- Protein processing by the placental protease, cathepsin P.Molecular human reproduction · 2009Article
- Profiling gene expression in human placentae of different gestational ages: an OPRU Network and UW SCOR Study.Reproductive sciences (Thousand Oaks, Calif.) · 2008Article
- Trophoblast differentiation during embryo implantation and formation of the maternal-fetal interface.The Journal of clinical investigation · 2004Review
- Trophoblast gene expression: transcription factors in the specification of early trophoblast.Reproductive biology and endocrinology : RB&E · 2004Review
- Amplified RNA degradation in T7-amplification methods results in biased microarray hybridizations.BMC genomics · 2003Article
- Gene expression profiling of embryo-derived stem cells reveals candidate genes associated with pluripotency and lineage specificity.Genome research · 2002Article
- Article
- Use of human cDNA microarrays for identification of differentially expressed genes in Atlantic salmon liver during Aeromonas salmonicida infection.Marine biotechnology (New York, N.Y.)Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The placenta is a highly specialized organ essential for embryonic growth and development. Here, we have applied cDNA subtraction between extraembryonic tissues of early- (day 7.5 of gestation) and late-stage embryos (day 17.5) to generate stage-specific cDNA pools that were used for screening of high-density mouse UniGene cDNA arrays containing 25,000 clones. A total of 638 clones were identified, 488 with the e7.5-specific probe and 150 with the e17.5-specific probe. Importantly, 363/638 (56.9%) of the hybridizing clones were not known to be expressed during placental development before. Differential regulation was confirmed by Northern blot and in situ hybridization for a total of 44/44 of positive clones. Thus, this combination of cDNA subtraction and array hybridization was highly successful for identification of genes expressed and regulated during placental development. These included growth factors and receptors, components of the transcriptional and translational machinery, cell cycle regulators, molecular chaperones, and cytoskeletal elements. The extensive in situ hybridization analysis revealed extraembryonic structures with a high density of differentially expressed genes, most strikingly the ectoplacental cone and the spongiotrophoblast. This large-scale identification of genes regulated during placentogenesis is extremely useful to further elucidate the molecular basis of extraembryonic development.
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