ArticleProceedings of the National Academy of Sciences of the United States of America2001
Parathyroid hormone regulates 25-hydroxyvitamin D(3)-24-hydroxylase mRNA by altering its stability.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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Who cites it
36 citing papers in PubMed, 90 citations in OpenAlex.
- Research on vitamin D metabolic regulation in the pathogenesis of related diseases.Frontiers in endocrinology · 2026Review
- Vitamin D and Acute Kidney Injury: A Reciprocal Relationship.Biomolecules · 2025Review
- Review
- Significant role of 1,25-dihydroxyvitamin D on serum calcium levels after total thyroidectomy: a prospective cohort study.Frontiers in endocrinology · 2024Article
- Molecular insights into mineralotropic hormone inter-regulation.Frontiers in endocrinology · 2023Review
- Regulation of 1 and 24 hydroxylation of vitamin D metabolites in the proximal tubule.Experimental biology and medicine (Maywood, N.J.) · 2022Review
- Vitamin D and Hypoxia: Points of Interplay in Cancer.Cancers · 2022Review
- Development of In Vitro and In Vivo Evaluation Systems for Vitamin D Derivatives and Their Application to Drug Discovery.International journal of molecular sciences · 2021Review
- Sclerostin Directly Stimulates Osteocyte Synthesis of Fibroblast Growth Factor-23.Calcified tissue international · 2021Article
- Influence of vitamin D treatment on functional expression of drug disposition pathways in human kidney proximal tubule cells during simulated uremia.Xenobiotica; the fate of foreign compounds in biological systems · 2021Article
- The Molecular Mechanisms by Which Vitamin D Prevents Insulin Resistance and Associated Disorders.International journal of molecular sciences · 2020Review
- A chromatin-based mechanism controls differential regulation of the cytochrome P450 geneThe Journal of biological chemistry · 2019Article
- Vitamin D Binding Protein and the Biological Activity of Vitamin D.Frontiers in endocrinology · 2019Review
- Exploring vitamin D metabolism and function in cancer.Experimental & molecular medicine · 2018Review
- Heterotrimeric G proteins in the control of parathyroid hormone actions.Journal of molecular endocrinology · 2017Review
- Roles of the kidney in the formation, remodeling and repair of bone.Journal of nephrology · 2016Review
- Article
- Compensatory Changes in Calcium Metabolism Accompany the Loss of Vitamin D Receptor (VDR) From the Distal Intestine and Kidney of Mice.Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research · 2016Article
- USP2-45 Is a Circadian Clock Output Effector Regulating Calcium Absorption at the Post-Translational Level.PloS one · 2016Article
- A High-Calcium and Phosphate Rescue Diet and VDR-Expressing Transgenes Normalize Serum Vitamin D Metabolite Profiles and Renal Cyp27b1 and Cyp24a1 Expression in VDR Null Mice.Endocrinology · 2015Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The up-regulation of the 25-hydroxyvitamin D(3)-24-hydroxylase by 1,25-dihydroxyvitamin D(3) [1,25(OH)(2)D(3)] is well established and occurs at the transcriptional level through two vitamin D response elements in the promoter of the gene. However, the mechanism of down-regulation of the 24-hydroxylase by parathyroid hormone (PTH) has not yet been elucidated. To study the mechanism of PTH action, we used AOK-B50 cells, a porcine kidney-cell line with stably transfected opossum PTH receptor in which both the 24-hydroxylase mRNA and activity are down-regulated by PTH. Cells dosed with 1,25(OH)(2)D(3) at 0 h, and subsequently at 0, 1, 2, or 4 h with 100 nM of PTH, showed levels of 24-hydroxylase mRNA equivalent to 72.6, 65.3, 57.2, and 37.1%, respectively, of the levels found in cells dosed with 1,25(OH)(2)D(3) only. All cells were collected 7 h after the initial 1,25(OH)(2)D(3) dose. This pattern of expression indicated that PTH does not act by repressing transcription but rather by making the mRNA for 24-hydroxylase susceptible to degradation. At least 1 h is required for PTH to act. Further RNA and protein syntheses are required for PTH to act. However, the sites and mechanism whereby PTH causes 24-hydroxylase mRNA degradation are unknown. Because the untranslated regions of genes can determine the stability of its transcripts, we studied the 5' untranslated region and the 3' untranslated region of the rat 24-hydroxylase gene by using reporter-gene strategy to identify possible PTH sites of action. None was found, suggesting that the destabilization site is elsewhere in the coding region.
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