ArticleJournal of bacteriology2001
Isolation and characterization of Escherichia coli tolC mutants defective in secreting enzymatically active alpha-hemolysin.
Article in Journal of bacteriology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed.
- Genomic correlates of tailocin sensitivity in Pseudomonas syringae.G3 (Bethesda, Md.) · 2025Article
- Review
- Review
- The Antibiotic Efflux Protein TolC Is a Highly Evolvable Target under Colicin E1 or TLS Phage Selection.Molecular biology and evolution · 2021Article
- Vibrio cholerae TolC Is Required for Expression of the ToxR Regulon.Infection and immunity · 2021Article
- Genomic Insights into Drug Resistance Determinants inMicroorganisms · 2021Article
- Structure, Assembly, and Function of Tripartite Efflux and Type 1 Secretion Systems in Gram-Negative Bacteria.Chemical reviews · 2021Review
- A Study on actinobacterial diversity of Hampoeil cave and screening of their biological activities.Saudi journal of biological sciences · 2019Article
- Type I Protein Secretion-Deceptively Simple yet with a Wide Range of Mechanistic Variability across the Family.EcoSal Plus · 2016Review
- AcrB, AcrD, and MdtABC multidrug efflux systems are involved in enterobactin export in Escherichia coli.PloS one · 2014Article
- Ehrlichia chaffeensis tandem repeat proteins and Ank200 are type 1 secretion system substrates related to the repeats-in-toxin exoprotein family.Frontiers in cellular and infection microbiology · 2011Article
- Mechanism and Function of the Outer Membrane Channel TolC in Multidrug Resistance and Physiology of Enterobacteria.Frontiers in microbiology · 2011Article
- AcrA suppressor alterations reverse the drug hypersensitivity phenotype of a TolC mutant by inducing TolC aperture opening.Molecular microbiology · 2010Article
- Biologic activities of the TolC-like protein of Neisseria meningitidis as assessed by functional complementation in Escherichia coli.Antimicrobial agents and chemotherapy · 2010Article
- An excretory function for the Escherichia coli outer membrane pore TolC: upregulation of marA and soxS transcription and Rob activity due to metabolites accumulated in tolC mutants.Journal of bacteriology · 2009Article
- MacAB is involved in the secretion of Escherichia coli heat-stable enterotoxin II.Journal of bacteriology · 2008Article
- Recombineering with tolC as a selectable/counter-selectable marker: remodeling the rRNA operons of Escherichia coli.Nucleic acids research · 2008Article
- The TolC-like protein of neisseria meningitidis is required for extracellular production of the repeats-in-toxin toxin FrpC but not for resistance to antimicrobials recognized by the Mtr efflux pump system.Infection and immunity · 2007Article
- Initial steps of colicin E1 import across the outer membrane of Escherichia coli.Journal of bacteriology · 2007Article
- Mutations in HlyD, part of the type 1 translocator for hemolysin secretion, affect the folding of the secreted toxin.Journal of bacteriology · 2005Article
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3 authors.
Funding
Abstract
This study describes the isolation and characterization of a unique class of TolC mutants that, under steady-state growth conditions, secreted normal levels of largely inactive alpha-hemolysin. Unlike the reduced activity in the culture supernatants, the cell-associated hemolytic activity in these mutants was identical to that in the parental strain, thus reflecting a normal intracellular toxin activation event. Treatment of the secreted toxin with guanidine hydrochloride significantly restored cytolytic activity, suggesting that the diminished activity may have been due to the aggregation or misfolding of the toxin molecules. Consistent with this notion, sedimentation and filtration analyses showed that alpha-hemolysin secreted from the mutant strain has a mass greater than that secreted from the parental strain. Experiments designed to monitor the time course of alpha-hemolysin release showed delayed appearance of toxin in the culture supernatant of the mutant strain, thus indicating a possible defect in alpha-hemolysin translocation or release. Eight different TolC substitutions displaying this toxin secretion defect were scattered throughout the protein, of which six localized in the periplasmically exposed alpha-helical domain, while the remaining two mapped within the outer membrane-embedded beta-barrel domain of TolC. A plausible model for the secretion of inactive alpha-hemolysin in these TolC mutants is discussed in the context of the recently determined three-dimensional structure of TolC.
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