ArticleJournal of bacteriology2001
Characterization of Pneumocystis carinii PHR1, a pH-regulated gene important for cell wall Integrity.
Article in Journal of bacteriology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
14 citing papers in PubMed, 30 citations in OpenAlex.
- Targeting β-glucans, vital components of theFrontiers in immunology · 2023Review
- The Beauveria bassiana Gas3 β-Glucanosyltransferase Contributes to Fungal Adaptation to Extreme Alkaline Conditions.Applied and environmental microbiology · 2018Article
- Characterization of N-Acetylglucosamine Biosynthesis in Pneumocystis species. A New Potential Target for Therapy.American journal of respiratory cell and molecular biology · 2017Article
- Evidence for a Pneumocystis carinii Flo8-like transcription factor: insights into organism adhesion.Medical microbiology and immunology · 2016Article
- Evidence for Proinflammatory β-1,6 Glucans in the Pneumocystis carinii Cell Wall.Infection and immunity · 2015Article
- Chitinases in Pneumocystis carinii pneumonia.Medical microbiology and immunology · 2012Article
- Pneumocystis carinii expresses an active Rtt109 histone acetyltransferase.American journal of respiratory cell and molecular biology · 2011Article
- Characterization of PCEng2, a {beta}-1,3-endoglucanase homolog in Pneumocystis carinii with activity in cell wall regulation.American journal of respiratory cell and molecular biology · 2010Article
- Characterization of the PcCdc42 small G protein from Pneumocystis carinii, which interacts with the PcSte20 life cycle regulatory kinase.American journal of physiology. Lung cellular and molecular physiology · 2010Article
- The Pneumocystis meiotic PCRan1p kinase exhibits unique temperature-regulated activity.American journal of respiratory cell and molecular biology · 2009Article
- Pneumocystis carinii exhibits a conserved meiotic control pathway.Infection and immunity · 2008Article
- Pneumocystis carinii cell wall biosynthesis kinase gene CBK1 is an environmentally responsive gene that complements cell wall defects of cbk-deficient yeast.Infection and immunity · 2004Article
- Lung epithelial cells and extracellular matrix components induce expression of Pneumocystis carinii STE20, a gene complementing the mating and pseudohyphal growth defects of STE20 mutant yeast.Infection and immunity · 2003Article
- Adaptation to environmental pH in Candida albicans and its relation to pathogenesis.Current genetics · 2003Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Pneumocystis carinii remains an important opportunistic fungal pathogen causing life-threatening pneumonia in patients with AIDS and malignancy. Currently, little is known about how the organism adapts to environmental stresses and maintains its cellular integrity. We recently discovered an open reading frame approximately 600 bp downstream of the region coding GSC-1, a gene mediating beta-glucan cell wall synthesis in P. carinii. The predicted amino acid sequence of this new gene, termed P. carinii PHR1, exhibited 38% homology to Saccharomyces cerevisiae GAS1, a glycosylphosphatidylinositol-anchored protein essential to maintaining cell wall integrity, and 37% homology to Candida albicans PHR1/PHR2, pH-responsive genes encoding proteins recently implicated in cross-linking beta-1,3- and beta-1,6-glucans. In view of its homology to these related fungal genes, the pH-dependent expression of P. carinii PHR1 was examined. As in C. albicans, P. carinii PHR1 expression was repressed under acidic conditions but induced at neutral and more alkaline pH. PHR1-related proteins have been implicated in glucan cell wall stability under various environmental conditions. Although difficulties with P. carinii culture and transformation have traditionally limited assessment of gene function in the organism itself, we have successfully used heterologous expression of P. carinii genes in related fungi to address functional correlates of P. carinii-encoded proteins. Therefore, the potential role of P. carinii PHR1 in cell wall integrity was examined by assessing its ability to rescue an S. cerevisiae gas1 mutant with absent endogenous Phr1p-like activity. Interestingly, P. carinii PHR1 DNA successfully restored proliferation of S. cerevisiae gas1 mutants under lethal conditions of cell wall stress. These results indicate that P. carinii PHR1 encodes a protein responsive to environmental pH and capable of mediating fungal cell wall integrity.
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