ArticleThe Journal of general physiology2001
Hidden Markov model analysis of intermediate gating steps associated with the pore gate of shaker potassium channels.
Article in The Journal of general physiology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 39 citations in OpenAlex.
- Distinct functional open states of the polymodal nociceptor TRPV1.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Evaluating sequential and allosteric activation models in IKs channels with mutated voltage sensors.The Journal of general physiology · 2024Article
- Opening of capsaicin receptor TRPV1 is stabilized equally by its four subunits.The Journal of biological chemistry · 2023Article
- The conformational wave in capsaicin activation of transient receptor potential vanilloid 1 ion channel.Nature communications · 2018Article
- Mechanosensitive gating of Kv channels.PloS one · 2015Review
- How to resolve microsecond current fluctuations in single ion channels: the power of beta distributions.Channels (Austin, Tex.) · 2015Review
- Multiple pore conformations driven by asynchronous movements of voltage sensors in a eukaryotic sodium channel.Nature communications · 2013Article
- Being flexible: the voltage-controllable activation gate of kv channels.Frontiers in pharmacology · 2012Article
- Using a five-state model for fitting amplitude histograms from MaxiK channels: beta-distributions reveal more than expected.European biophysics journal : EBJ · 2009Article
- Molecular template for a voltage sensor in a novel K+ channel. III. Functional reconstitution of a sensorless pore module from a prokaryotic Kv channel.The Journal of general physiology · 2008Article
- Four-mode gating model of fast inactivation of sodium channel Nav1.2a.Pflugers Archiv : European journal of physiology · 2008Article
- Direct analysis of cooperativity in multisubunit allosteric proteins.Proceedings of the National Academy of Sciences of the United States of America · 2008Article
- Tl+-induced micros gating of current indicates instability of the MaxiK selectivity filter as caused by ion/pore interaction.The Journal of general physiology · 2008Article
- Ion binding in the open HCN pacemaker channel pore: fast mechanisms to shape "slow" channels.The Journal of general physiology · 2008Article
- Time-dependent molecular memory in single voltage-gated sodium channel.The Journal of membrane biology · 2007Article
- Saturation and microsecond gating of current indicate depletion-induced instability of the MaxiK selectivity filter.The Journal of general physiology · 2007Article
- Thermosensitive TRPV channel subunits coassemble into heteromeric channels with intermediate conductance and gating properties.The Journal of general physiology · 2007Article
- Strengths and limits of Beta distributions as a means of reconstructing the true single-channel current in patch clamp time series with fast gating.The Journal of membrane biology · 2006Article
- The power of two-dimensional dwell-time analysis for model discrimination, temporal resolution, multichannel analysis and level detection.The Journal of membrane biology · 2006Article
- The cooperative voltage sensor motion that gates a potassium channel.The Journal of general physiology · 2005Article
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
Cooperativity among the four subunits helps give rise to the remarkable voltage sensitivity of Shaker potassium channels, whose open probability changes tenfold for a 5-mV change in membrane potential. The cooperativity in these channels is thought to arise from a concerted structural transition as the final step in opening the channel. Recordings of single-channel ionic currents from certain other channel types, as well as our previous recordings from T442S mutant Shaker channels, however, display intermediate conductance levels in addition to the fully open and closed states. These sublevels might represent stepwise, rather than concerted, transitions in the final steps of channel activation. Here, we report a similar fine structure in the closing transitions of Shaker channels lacking the mutation. Describing the deactivation time course with hidden Markov models, we find that two subconductance levels are rapidly traversed during most closing transitions of chimeric, high conductance Shaker channels. The lifetimes of these levels are voltage-dependent, with maximal values of 52 and 22 micros at -100 mV, and the voltage dependences of transitions among these states suggest that they arise from equivalent conformational changes occurring in individual subunits. At least one subconductance level is found to be traversed in normal conductance Shaker channels. We speculate that voltage-dependent conformational changes in the subunits give rise to changes in a "pore gate" associated with the selectivity filter region of the channel, producing the subconductance states. As a control for the hidden Markov analysis, we applied the same procedures to recordings of the recovery from N-type inactivation in Shaker channels. These transitions are found to be instantaneous in comparison.
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