ArticleThe Journal of cell biology2001
Evidence that the entire Golgi apparatus cycles in interphase HeLa cells: sensitivity of Golgi matrix proteins to an ER exit block.
Article in The Journal of cell biology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
42 citing papers in PubMed, 97 citations in OpenAlex.
- Plant Golgi and GECCO: The Universality of ER-Golgi Interface.Sub-cellular biochemistry · 2026Review
- Trafficking and localization of Golgi-residentFrontiers in plant science · 2025Review
- Fine-tuning cell organelle dynamics during mitosis by small GTPases.Frontiers of medicine · 2022Review
- Photoactivatable Hsp47: A Tool to Regulate Collagen Secretion and Assembly.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2019Article
- ER trapping reveals Golgi enzymes continually revisit the ER through a recycling pathway that controls Golgi organization.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Sit4p/PP6 regulates ER-to-Golgi traffic by controlling the dephosphorylation of COPII coat subunits.Molecular biology of the cell · 2013Article
- Photohighlighting approaches to access membrane dynamics of the Golgi apparatus.Methods in cell biology · 2013Article
- Fluorescent microscopy as a tool to elucidate dysfunction and mislocalization of Golgi glycosyltransferases in COG complex depleted mammalian cells.Methods in molecular biology (Clifton, N.J.) · 2013Article
- Identification of a functional domain within the p115 tethering factor that is required for Golgi ribbon assembly and membrane trafficking.Journal of cell science · 2012Article
- Live-cell assays to identify regulators of ER-to-Golgi trafficking.Traffic (Copenhagen, Denmark) · 2012Article
- Irradiation-induced protein inactivation reveals Golgi enzyme cycling to cell periphery.Journal of cell science · 2012Article
- Sequential depletion and acquisition of proteins during Golgi stack disassembly and reformation.Traffic (Copenhagen, Denmark) · 2010Article
- Spatial regulation of Golgi phosphatidylinositol-4-phosphate is required for enzyme localization and glycosylation fidelity.Traffic (Copenhagen, Denmark) · 2010Article
- Discrete, continuous, and stochastic models of protein sorting in the Golgi apparatus.Physical review. E, Statistical, nonlinear, and soft matter physics · 2010Article
- Conserved molecular mechanisms underlying homeostasis of the Golgi complex.International journal of cell biology · 2010Article
- Membrane traffic within the Golgi apparatus.Annual review of cell and developmental biology · 2009Review
- The membrane dynamics of pexophagy are influenced by Sar1p in Pichia pastoris.Molecular biology of the cell · 2008Article
- Simulated de novo assembly of golgi compartments by selective cargo capture during vesicle budding and targeted vesicle fusion.Biophysical journal · 2008Article
- Golgi regeneration after brefeldin A treatment in BY-2 cells entails stack enlargement and cisternal growth followed by division.Plant physiology · 2007Article
- Rab6 regulates both ZW10/RINT-1 and conserved oligomeric Golgi complex-dependent Golgi trafficking and homeostasis.Molecular biology of the cell · 2007Article
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4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We tested whether the entire Golgi apparatus is a dynamic structure in interphase mammalian cells by assessing the response of 12 different Golgi region proteins to an endoplasmic reticulum (ER) exit block. The proteins chosen spanned the Golgi apparatus and included both Golgi glycosyltransferases and putative matrix proteins. Protein exit from ER was blocked either by microinjection of a GTP-restricted Sar1p mutant protein in the presence of a protein synthesis inhibitor, or by plasmid-encoded expression of the same dominant negative Sar1p. All Golgi region proteins examined lost juxtanuclear Golgi apparatus-like distribution as scored by conventional and confocal fluorescence microscopy in response to an ER exit block, albeit with a differential dependence on Sar1p concentration. Redistribution of GalNAcT2 was more sensitive to low Sar1p(dn) concentrations than giantin or GM130. Redistribution was most rapid for p27, COPI, and p115. Giantin, GM130, and GalNAcT2 relocated with approximately equal kinetics. Distinct ER accumulation could be demonstrated for all integral membrane proteins. ER-accumulated Golgi region proteins were functional. Photobleaching experiments indicated that Golgi-to-ER protein cycling occurred in the absence of any ER exit block. We conclude that the entire Golgi apparatus is a dynamic structure and suggest that most, if not all, Golgi region-integral membrane proteins cycle through ER in interphase cells.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.