ArticleBMC biochemistry2001
Beta1 integrins show specific association with CD98 protein in low density membranes.
Article in BMC biochemistry, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
27 citing papers in PubMed, 53 citations in OpenAlex.
- Identification by proximity labeling of novel lipidic and proteinaceous potential partners of the dopamine transporter.Cellular and molecular life sciences : CMLS · 2021Article
- The heavy chain of 4F2 antigen promote prostate cancer progression via SKP-2.Scientific reports · 2021Article
- Heteromeric Solute Carriers: Function, Structure, Pathology and Pharmacology.Advances in experimental medicine and biology · 2021Review
- Molecular mapping of transmembrane mechanotransduction through the β1 integrin-CD98hc-TRPV4 axis.Journal of cell science · 2020Article
- Overexpression of CD98 in intestinal epithelium dysregulates miRNAs and their targeted proteins along the ileal villus-crypt axis.Scientific reports · 2018Article
- L-type amino acid transport and cancer: targeting the mTORC1 pathway to inhibit neoplasia.American journal of cancer research · 2015Review
- New insights into the DT40 B cell receptor cluster using a proteomic proximity labeling assay.The Journal of biological chemistry · 2014Article
- Intestinal epithelial CD98 directly modulates the innate host response to enteric bacterial pathogens.Infection and immunity · 2013Article
- CD98hc (SLC3A2) regulation of skin homeostasis wanes with age.The Journal of experimental medicine · 2013Article
- CD98 at the crossroads of adaptive immunity and cancer.Journal of cell science · 2012Review
- Article
- Release of membrane-bound vesicles and inhibition of tumor cell adhesion by the peptide Neopetrosiamide A.PloS one · 2010Article
- G alpha 12 inhibits alpha2 beta1 integrin-mediated Madin-Darby canine kidney cell attachment and migration on collagen-I and blocks tubulogenesis.Molecular biology of the cell · 2009Article
- Analysis of the gamma-secretase interactome and validation of its association with tetraspanin-enriched microdomains.Nature cell biology · 2009Article
- Combining results from lectin affinity chromatography and glycocapture approaches substantially improves the coverage of the glycoproteome.Molecular & cellular proteomics : MCP · 2009Article
- Article
- Intestinal epithelial CD98: an oligomeric and multifunctional protein.Biochimica et biophysica acta · 2008Review
- CD98 activation increases surface expression and clusteringof beta1 integrins in MCF-7 cells through FAK/Src- and cytoskeleton-independent mechanisms.Experimental & molecular medicine · 2008Article
- Integrin alphaVbeta3 Binds to the RGD motif of glycoprotein B of Kaposi's sarcoma-associated herpesvirus and functions as an RGD-dependent entry receptor.Journal of virology · 2008Article
- Extracellular interaction between hCD98 and the PDZ class II domain of hCASK in intestinal epithelia.The Journal of membrane biology · 2007Article
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 2 countries.
Funding
Abstract
backgroundThe CD98 (4F2, FRP-1) is a widely expressed cell surface protein heterodimer composed of a glycosylated heavy chain and a non-glycosylated light chain. Originally described as a T cell activation antigen, it was later shown to function in amino acid transport, cell fusion and homotypic cell aggregation. Several lines of evidence suggest its functional interaction with integrins but the biochemical basis for this interaction has been unclear.
resultsWe demonstrate that CD98 constitutively and specifically associates with beta1 integrins (alpha2beta1,alpha3beta1, alpha5beta1 and alpha6beta1), but minimally with alpha4beta1. Integrin-CD98 association was established by reciprocal immunoprecipitation experiments, and confirmed by CD98-induced clustering of alpha3beta1 but not alpha4beta1 on the surface of rhabdomyosarcoma cells. Integrin-CD98 association is independent of the alpha subunit cytoplasmic tail, is maintained in alpha3beta1 ligand-interaction deficient mutants, and is not inhibited by EDTA. Within the CD98 heavy chain, a C109S mutation (but not a C330S mutation) caused a loss of beta1 integrin association. The same C109S mutation also caused a loss of CD98 light chain association. Importantly, CD98 associated selectively with beta1 integrins present in low density "light membrane" fractions on a sucrose gradient. CD98 was not present in dense fractions that contained the majority of beta1 integrins. Notably, the C109S mutant of CD98, that did not associate with beta1 integrins, showed also a reduced localization into light membrane fractions.
conclusionsWe demonstrate that CD98 association with beta1 integrins is specific, occurs in the context of low density membranes, and may require the CD98 light chain.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.