ArticleThe Biochemical journal2001
Fatty-acid-binding proteins do not protect against induced cytotoxicity in a kidney cell model.
Article in The Biochemical journal, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 26 citations in OpenAlex.
- Fatty acid-binding protein 5 deficiency impairs alveolar macrophage function and metabolism.Journal of lipid research · 2026Article
- Macrophage programming is regulated by a cooperative interaction between fatty acid binding protein 5 and peroxisome proliferator-activated receptor γ.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2022Article
- Expression of fatty-acid-binding protein 5 in intrahepatic and extrahepatic cholangiocarcinoma: the possibility of different energy metabolisms in anatomical location.Medical molecular morphology · 2020Article
- Mice Lacking Fatty Acid-Binding Protein 5 Are Resistant to Listeria monocytogenes.Journal of innate immunity · 2019Article
- Comparative transcriptome analysis of epithelial and fiber cells in newborn mouse lenses with RNA sequencing.Molecular vision · 2014Article
- FABP5 deficiency enhances susceptibility to H1N1 influenza A virus-induced lung inflammation.American journal of physiology. Lung cellular and molecular physiology · 2013Article
- Cigarette smoke decreases airway epithelial FABP5 expression and promotes Pseudomonas aeruginosa infection.PloS one · 2013Article
- Urinary liver-type fatty acid-binding protein predicts progression to nephropathy in type 1 diabetic patients.Diabetes care · 2010Article
- Tubular and glomerular injury in diabetes and the impact of ACE inhibition.Diabetes care · 2009Article
- Expression of E-FABP in PC12 cells increases neurite extension during differentiation: involvement of n-3 and n-6 fatty acids.Journal of neurochemistry · 2008Article
- Liver-type fatty acid-binding protein attenuates renal injury induced by unilateral ureteral obstruction.The American journal of pathology · 2006Article
- Urinary excretion of fatty acid-binding protein reflects stress overload on the proximal tubules.The American journal of pathology · 2004Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Intracellular accumulation of fatty acids (FAs) is a well-described consequence of renal ischaemia and may lead to lethal cell injury. Fatty-acid-binding proteins (FABPs) are small cytosolic proteins with high affinity for FAs. They may protect vital cellular functions by binding to and promoting the metabolism of FAs, thereby reducing their intracellular concentration. In this study we investigated the putative cytoprotective role of FABPs in a Madin-Darby canine kidney (MDCK) cell model for renal damage. We studied the effects of transfection with cDNA encoding heart FABP, adipocyte FABP or liver FABP on cytotoxicity induced by chemical anoxia or FAs. Transfection of MDCK type II cells with these cDNA types caused a 5-20-fold increase in FABP content, but did not change the rate or extent of palmitate uptake. After 1 h of incubation with KCN, all cell types showed reduced viability and cellular ATP content and an intracellular accumulation of non-esterified FAs. High extracellular concentrations of oleate, but not palmitate, caused a markedly decreased cell viability and cellular ATP content. Oleate accumulated in non-esterified form in these cells. Simultaneous addition of glucose ameliorated the damaging effects of KCN or oleate, indicating that glycolytic ATP could substitute for uncoupled oxidative phosphorylation. No significant differences in the effects of chemical anoxia or oleate were observed between non-transfected, mock-transfected and FABP-cDNA-transfected cells. Non-esterified FA accumulation was not reduced in any of the FABP-cDNA-transfected cell lines. In conclusion, our data do not provide evidence for a cytoprotective role of FABP in this kidney cell model.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.