ArticleThe Biochemical journal2001
Characterization of zebrafish caspase-3 and induction of apoptosis through ceramide generation in fish fathead minnow tailbud cells and zebrafish embryo.
Article in The Biochemical journal, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 139 citations in OpenAlex.
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- Oxidative stress induced by hydrogen peroxide disrupts zebrafish visual development by altering apoptosis, antioxidant and estrogen related genes.Scientific reports · 2024Article
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- Zebrafish Klf11b is Required to Maintain Cell Viability by Inhibiting p53-Mediated Apoptosis.Development & reproduction · 2022Article
- Effect of Water-Pipe Smoking on the Normal Development of Zebrafish.International journal of environmental research and public health · 2021Article
- Expression Analysis of the Caspase10 from Olive Flounder (Development & reproduction · 2020Article
- Characterization of the responses of the caspase 2, 3, 6 and 8 genes to immune challenges and extracellular ATP stimulation in the Japanese flounder (Paralichthys olivaceus).BMC veterinary research · 2019Article
- Identification of Five Key Genes Involved in Intrinsic Apoptotic Pathway From Yellow CatfishFrontiers in physiology · 2019Article
- Disassembly of dying cells in diverse organisms.Cellular and molecular life sciences : CMLS · 2019Review
- Starfish Apaf-1 activates effector caspase-3/9 upon apoptosis of aged eggs.Scientific reports · 2018Article
- Phage display and structural studies reveal plasticity in substrate specificity of caspase-3a from zebrafish.Protein science : a publication of the Protein Society · 2016Article
- Identification of benzopyrone as a common structural feature in compounds with anti-inflammatory activity in a zebrafish phenotypic screen.Disease models & mechanisms · 2016Article
- Stress-induced ceramide generation and apoptosis via the phosphorylation and activation of nSMase1 by JNK signaling.Cell death and differentiation · 2015Article
- Development of Cre-loxP technology in zebrafish to study the regulation of fish reproduction.Fish physiology and biochemistry · 2013Article
- Local caspase activation interacts with Slit-Robo signaling to restrict axonal arborization.The Journal of cell biology · 2013Article
- In vitro selection of proteins with desired characteristics using mRNA-display.Methods (San Diego, Calif.) · 2013Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Caspase-3 was cloned from zebrafish embryos and its properties were characterized to identify the biological implications of caspase in embryogenesis and apoptosis in zebrafish, which is a model organism in vertebrate developmental biology and genetics. The predicted amino acid sequence, totalling 282 amino acid residues, consisted of the prodomain and large and small subunits. Phylogenetic analysis showed that the cloned zebrafish caspase was a member of the caspase-3 subfamily with approx. 60% identity with caspase-3 from Xenopus, chicken and mammals. In addition, recombinant zebrafish caspase hydrolysed acetyl-Asp-Glu-Val-Asp-4-methyl-coumaryl-7-amide, and exhibited similar substrate specificity to the mammalian caspase-3 subfamily. Therefore this caspase was designated zebrafish caspase-3. Overexpression of zebrafish caspase-3 induced apoptosis and increased ceramide levels in fish fathead minnow tailbud cells and zebrafish embryos. Both ceramide generation and apoptosis induction were inhibited by treatment with a caspase inhibitor, benzyloxycarbonyl-Asp-Glu-Val-Asp-fluoromethylketone. Moreover, zebrafish caspase-3 mRNA was present in early embryos up to the 1000-cell stage as a maternal factor, and was then expressed throughout the body after the gastrula stage by zygotic expression. These findings indicate that the isolated caspase-3 plays an important role in the induction of ceramide generation as well as apoptosis in fish cells and the zebrafish embryo, and suggest that caspase-3 functions as a modulator of the pro-apoptotic signal in development.
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